Subgingival microbiota in healthy, well‐maintained elder and periodontitis subjects
- 1 May 1998
- journal article
- research article
- Published by Wiley in Journal of Clinical Periodontology
- Vol. 25 (5), 346-353
- https://doi.org/10.1111/j.1600-051x.1998.tb02454.x
Abstract
This investigation compared the site prevalence of 40 subgingival species in 30 periodontally healthy (mean age 36±9 years). 35 elders with a well‐maintained periodontium (mean age 77±5) and 138 adult periodontitis subjects (mean age 46± 11). Subgingival plaque samples were taken from the mesial aspect of each tooth (up to 28 samples) in the 203 subjects at baseline. The presence and levels of 40 subgingival taxa were determined in 5003 plaque samples using whole genomic DNA probes and checkerboard DNA‐DNA hybridization. Clinical assessments including dichotomous measures of gingival redness, bleeding on probing, plaque accumulation and suppuration, as well as duplicate measures of pocket depth and attachment level, were made at 6 sites per tooth. The % of sites colonized by each species (prevalence) was computed for each subject. Differences in prevalence and levels among groups were sought using the Kruskal‐Wallis test. Commonly detected species, such as Actinomyces naeslundii genospecies 2, Streptococcus sanguis and Streptococcus oralis did not differ significantly among subject groups. After adjusting for multiple comparisons, 4 species were significantly elevated and at greater prevalence in the periodontitis group. Mean % of sites (±SEM) colonized by Bacteroides forsythus was 10±3, 12±2 and 40±2 (pB. forsythus was detected at ≥5% of sampled sites. Mean prevalence for Porphyromonas gingivalis in healthy, elder and periodontitis subjects was 4±2, 5±2 and 23±2 respectively (pTreponema denticola 12±4, 10±3 and 30±2 (pSelenomonas noxia 6±2, 7±2 and 19±2 (pB. forsythus, P. gingivalis, T. denticola and S. noxia in adult periodontitis.Keywords
This publication has 29 references indexed in Scilit:
- Trypsin-like activity levels of Treponema denticola and Porphyromonas gingivalis in adults with periodontitisJournal of Clinical Periodontology, 1994
- Natural distribution of 5 bacteria associated with periodontal diseaseJournal of Clinical Periodontology, 1993
- Porphyromonas gingivalis invades oral epithelial cells in vitroJournal of Periodontal Research, 1993
- Treponema denticola and Porphyromonas gingivalis as Prognostic Markers Following Periodontal TreatmentThe Journal of Periodontology, 1992
- Bacterial synergy of Treponema denticola and Porphyromonas gingivalis in a multinational populationOral Microbiology and Immunology, 1992
- Relation of counts of microbial species to clinical status at the sampled siteJournal of Clinical Periodontology, 1991
- Subgingival Microflora of Advanced Periodontitis in the Dominican RepublicThe Journal of Periodontology, 1991
- Clinical, microbiological and immunological characteristics of subjects with “refractory” periodontal diseaseJournal of Clinical Periodontology, 1991
- Prevalence and antimicrobial susceptibility of Enterobacteriaceae, Pseudomonadaceae and Acinetobacter in human periodontitisOral Microbiology and Immunology, 1990
- Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall*Journal of Periodontal Research, 1989