Investigation of Natural Phosphatidylcholine Sources: Separation and Identification by Liquid Chromatography−Electrospray Ionization−Tandem Mass Spectrometry (LC−ESI−MS2) of Molecular Species
- 22 June 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 57 (14), 6014-6020
- https://doi.org/10.1021/jf900903e
Abstract
This study is a contribution to the exploration of natural phospholipid (PL) sources rich in long-chain polyunsaturated fatty acids (LC-PUFAs) with nutritional interest. Phosphatidylcholines (PCs) were purified from total lipid extracts of different food matrices, and their molecular species were separated and identified by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS(2)). Fragmentation of lithiated adducts allowed for the identification of fatty acids linked to the glycerol backbone. Soy PC was particularly rich in species containing essential fatty acids, such as (18:2-18:2)PC (34.0%), (16:0-18:2)PC (20.8%), and (18:1-18:2)PC (16.3%). PC from animal sources (ox liver and egg yolk) contained major molecular species, such as (16:0-18:2)PC, (16:0-18:1)PC, (18:0-18:2)PC, or (18:0-18:1)PC. Finally, marine source (krill oil), which was particularly rich in (16:0-20:5)PC and (16:0-22:6)PC, appeared to be an interesting potential source for food supplementation with LC-PUFA-PLs, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA).This publication has 19 references indexed in Scilit:
- Polar lipids: n-3 PUFA carriers for membranes and brain: nutritional interest and emerging processesOléagineux, Corps gras, Lipides, 2007
- Nutraceutical and Specialty Lipids and their Co-ProductsPublished by Taylor & Francis Ltd ,2006
- High performance liquid chromatography-tandem mass spectrometry of phospholipid molecular species in eggs from hens fed diets enriched in seal blubber oilJournal of Chromatography A, 2005
- Long-Chain (n-3) Polyunsaturated Fatty Acids Are More Efficient than α-Linolenic Acid in Improving Electroretinogram Responses of Puppies Exposed during Gestation, Lactation, and WeaningJournal of Nutrition, 2005
- Analysis of Phospho- and Sphingolipids in Dairy Products by a New HPLC MethodJournal of Dairy Science, 2005
- Efficacy of Dietary Arachidonic Acid Provided as Triglyceride or Phospholipid as Substrates for Brain Arachidonic Acid Accretion in Baboon NeonatesPediatric Research, 2002
- Feeding Infant Piglets Formula with Long-Chain Polyunsaturated Fatty Acids as Triacylglycerols or Phospholipids Influences the Distribution of These Fatty Acids in Plasma Lipoprotein FractionsJournal of Nutrition, 2001
- HEALTH BENEFITS OF DOCOSAHEXAENOIC ACID (DHA)Pharmacological Research, 1999
- Visual Acuity, Erythrocyte Fatty Acid Composition, and Growth in Term Infants Fed Formulas with Long Chain Polyunsaturated Fatty Acids for One YearPediatric Research, 1997
- Feeding formula without arachidonic acid and docosahexaenoic acid has no effect on preferential looking acuity or recognition memory in healthy full-term infants at 9 mo of ageThe American Journal of Clinical Nutrition, 1996