Analysis of Phospho- and Sphingolipids in Dairy Products by a New HPLC Method
- 1 February 2005
- journal article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 88 (2), 482-488
- https://doi.org/10.3168/jds.s0022-0302(05)72710-7
Abstract
Dairy phospho- and sphingolipids are gaining interest due to their nutritional and technological properties. A new HPLC method, using an evaporative laser light-scattering detector, was developed, which enabled excellent separation of glucosylceramide, lactosylceramide, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine in less than 21 min, including the regeneration of the column. No loss of column performance was observed after 1500 runs because an acid buffer was used. The output signal of the evaporative laser light scattering detector was highly dependent of the flow of the carrier gas and the temperature of the nebulizer, and was maximized by means of a response surface experimental design. Finally, raw milk, cream, butter, buttermilk, Cheddar whey, quarg, and Cheddar cheese were analyzed for their polar lipid content. The absolute values varied substantially (0.018 to 0.181 g/100 g of product). Significant differences were found in the relative content of each polar lipid class among the analyzed products.Keywords
This publication has 14 references indexed in Scilit:
- Bovine milk fat components inhibit food-borne pathogensInternational Dairy Journal, 2002
- Apparent ζ-potential as a tool to assess mechanical damages to the milk fat globule membraneColloids and Surfaces B: Biointerfaces, 2002
- Phospholipids for functional foodEuropean Journal of Lipid Science and Technology, 2001
- A REVIEW OF PHOSPHATIDYLSERINE PHARMACOLOGICAL AND CLINICAL EFFECTS. IS PHOSPHATIDYLSERINE A DRUG FOR THE AGEING BRAIN?Pharmacological Research, 1996
- Quantitative analysis of phospholipids by HPLC with a light scattering evaporating detector – application to raw materials for cosmetic useJournal of High Resolution Chromatography, 1990
- A new rapid method for phospholipid separation by high-performance liquid chromatography with light-scattering detectionJournal of Chromatography B: Biomedical Sciences and Applications, 1989
- Phospholipids in milk and dairy productsInternational Journal of Dairy Technology, 1987
- Lipid extraction of tissues with a low-toxicity solventAnalytical Biochemistry, 1978
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959