Genetic and Biochemical Characterization of a Chromosome-Encoded Carbapenem-Hydrolyzing Ambler Class D β-Lactamase fromShewanella algae
- 1 May 2004
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 48 (5), 1670-5
- https://doi.org/10.1128/aac.48.5.1670-1675.2004
Abstract
A chromosome-encoded β-lactamase gene fromShewanella algaeclinical isolate KB-1 was cloned and expressed inEscherichia coli. It encoded the Ambler class D enzyme OXA-55, sharing less than 55% identity with any other oxacillinases. Although conferring a narrow-spectrum β-lactam resistance phenotype, OXA-55 had carbapenem-hydrolyzing activity that mirrored the reduced susceptibility to imipenem observed inS. algaeKB-1. Very similar oxacillinases were found in otherS. algaeisolates.Keywords
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