Isolation of Multidrug-Resistant Helicobacter pylori from Wild Houseflies Musca domestica with a New Perspective for the Treatment
- 27 December 2022
- journal article
- research article
- Published by Mary Ann Liebert Inc in Vector-Borne and Zoonotic Diseases
- Vol. 23 (2), 63-74
- https://doi.org/10.1089/vbz.2022.0033
Abstract
Background: High frequency of Helicobacter pylori infection and the unknown mode of transmission prompted us to investigate H. pylori–wild housefly relationship. H. pylori causes chronic gastritis, peptic ulcers, and stomach cancer. H. pylori persists in the gut of the experimentally infected houseflies. The existence of H. pylori strains isolated from wild houseflies, on the other hand, has never been documented. Methods: In this study, 902 wild houseflies from different sites were identified as Musca domestica, then 60 flies were screened by traditional microbiological techniques and H. pylori-specific 16S rRNA gene. The antibiotic resistance (ART) was investigated phenotypically. Wild housefly gut bacterial isolates were further evaluated genotypically to have 23S rRNA gene mutation related to clarithromycin resistance. To find efficient therapeutic alternatives, the potency of three plant extracts (garlic, ginger, and lemon) and the wasp, Vespa orientalis venom was evaluated against H. pylori. The cytotoxic effect of the crude wasp venom, the most potent extract, against Vero and Colon cancer (Caco2) cell lines was investigated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Results: All isolates from houseflies were positive. The isolated bacteria have variable resistance to frequently used antibiotics in all isolates. Minimum inhibitory concentration values of 15.625 mg/mL for both ginger and lemon extracts, 7.8125 mg/mL for garlic extract, and 0.0313 mg/mL for wasp venom were recorded. Wasp venom has the most potent antibacterial activity compared with the four antibiotics that are currently used in therapies against H. pylori. Conclusion: We conclude that wild houseflies can play a role in disseminating H. pylori. The housefly gut may be a suitable environment for the horizontal transfer of ART genes among its associated microbiome and H. pylori. Wasp venom proved its potential activity as a new and effective anti-H. pylori drug for both therapeutic and preventative usage.Keywords
This publication has 51 references indexed in Scilit:
- Foodborne Disease Prevention and Broiler Chickens with ReducedCampylobacterInfectionEmerging Infectious Diseases, 2013
- Antibacterial effect of Allium sativum cloves and Zingiber officinale rhizomes against multiple-drug resistant clinical pathogensAsian Pacific Journal of Tropical Biomedicine, 2012
- Circulating interleukin-27 levels in Helicobacter pylori-infected patients with gastric or duodenal ulcers, independent of the bacterial cytotoxin-associated gene A virulence factorJournal of Digestive Diseases, 2011
- In vitro antibacterial activity of some Iranian medicinal plant extracts against Helicobacter pyloriNatural Product Research, 2011
- Resident Microbiota of the Gypsy Moth Midgut Harbors Antibiotic Resistance DeterminantsDNA and Cell Biology, 2009
- Temporal and Spatial Fate of GFP-Expressing Motile and NonmotileAeromonas hydrophilain the House Fly Digestive TractJournal of Medical Entomology, 2009
- Persistence of Helicobacter pylori in Heterotrophic Drinking-Water BiofilmsApplied and Environmental Microbiology, 2008
- Inhibitory activity of Thai condiments on pandemic strain of Vibrio parahaemolyticusFood Microbiology, 2007
- Ecology of Antibiotic Resistance Genes: Characterization of Enterococci from Houseflies Collected in Food SettingsApplied and Environmental Microbiology, 2006
- Universal High-Level Primary Metronidazole Resistance in Helicobacter pylori Isolated from Children in EgyptJournal of Clinical Microbiology, 2004