Paradoxical DNA Repair and Peroxide Resistance Gene Conservation in Bacillus pumilus SAFR-032
Open Access
- 26 September 2007
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 2 (9), e928
- https://doi.org/10.1371/journal.pone.0000928
Abstract
Bacillus spores are notoriously resistant to unfavorable conditions such as UV radiation, γ-radiation, H2O2, desiccation, chemical disinfection, or starvation. Bacillus pumilus SAFR-032 survives standard decontamination procedures of the Jet Propulsion Lab spacecraft assembly facility, and both spores and vegetative cells of this strain exhibit elevated resistance to UV radiation and H2O2 compared to other Bacillus species. The genome of B. pumilus SAFR-032 was sequenced and annotated. Lists of genes relevant to DNA repair and the oxidative stress response were generated and compared to B. subtilis and B. licheniformis. Differences in conservation of genes, gene order, and protein sequences are highlighted because they potentially explain the extreme resistance phenotype of B. pumilus. The B. pumilus genome includes genes not found in B. subtilis or B. licheniformis and conserved genes with sequence divergence, but paradoxically lacks several genes that function in UV or H2O2 resistance in other Bacillus species. This study identifies several candidate genes for further research into UV and H2O2 resistance. These findings will help explain the resistance of B. pumilus and are applicable to understanding sterilization survival strategies of microbes.This publication has 70 references indexed in Scilit:
- Analysis of Temporal Gene Expression duringBacillus subtilisSpore Germination and OutgrowthJournal of Bacteriology, 2007
- Role of DNA Repair by Nonhomologous-End Joining in Bacillus subtilis Spore Resistance to Extreme Dryness, Mono- and Polychromatic UV, and Ionizing RadiationJournal of Bacteriology, 2007
- Genome analysis of DNA repair genes in the alpha proteobacterium Caulobacter crescentusBMC Microbiology, 2007
- Enhanced automated function prediction using distantly related sequences and contextual association by PFPProtein Science, 2006
- The Forespore Line of Gene Expression in Bacillus subtilisJournal of Molecular Biology, 2006
- TCDB: the Transporter Classification Database for membrane transport protein analyses and informationNucleic Acids Research, 2006
- The Atlas Genome Assembly SystemGenome Research, 2004
- Spore UV and Acceleration Resistance of Endolithic Bacillus pumilus and Bacillus subtilis Isolates Obtained from Sonoran Desert Basalt: Implications for LithopanspermiaAstrobiology, 2003
- Improved microbial gene identification with GLIMMERNucleic Acids Research, 1999
- Inducible DNA repair and differentiation in Bacillus subtilis: interactions between global regulonsMolecular Microbiology, 1992