Role of DNA Repair by Nonhomologous-End Joining in Bacillus subtilis Spore Resistance to Extreme Dryness, Mono- and Polychromatic UV, and Ionizing Radiation
Open Access
- 15 April 2007
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (8), 3306-3311
- https://doi.org/10.1128/jb.00018-07
Abstract
The role of DNA repair by nonhomologous-end joining (NHEJ) in spore resistance to UV, ionizing radiation, and ultrahigh vacuum was studied in wild-type and DNA repair mutants ( recA , splB , ykoU , ykoV , and ykoU ykoV mutants) of Bacillus subtilis . NHEJ-defective spores with mutations in ykoU , ykoV , and ykoU ykoV were significantly more sensitive to UV, ionizing radiation, and ultrahigh vacuum than wild-type spores, indicating that NHEJ provides an important pathway during spore germination for repair of DNA double-strand breaks.Keywords
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