Generation of cerebral organoids from human pluripotent stem cells
Top Cited Papers
Open Access
- 30 September 2014
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Protocols
- Vol. 9 (10), 2329-2340
- https://doi.org/10.1038/nprot.2014.158
Abstract
Human brain development exhibits several unique aspects, such as increased complexity and expansion of neuronal output, that have proven difficult to study in model organisms. As a result, in vitro approaches to model human brain development and disease are an intense area of research. Here we describe a recently established protocol for generating 3D brain tissue, so-called cerebral organoids, which closely mimics the endogenous developmental program. This method can easily be implemented in a standard tissue culture room and can give rise to developing cerebral cortex, ventral telencephalon, choroid plexus and retinal identities, among others, within 1-2 months. This straightforward protocol can be applied to developmental studies, as well as to the study of a variety of human brain diseases. Furthermore, as organoids can be maintained for more than 1 year in long-term culture, they also have the potential to model later events such as neuronal maturation and survival.This publication has 42 references indexed in Scilit:
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