Selecting Aptamers for a Glycoprotein through the Incorporation of the Boronic Acid Moiety
- 3 September 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 130 (38), 12636-12638
- https://doi.org/10.1021/ja801510d
Abstract
The first general method for the selection of boronic acid−based aptamers (boronolectins) that allows for glycan substructure focusing is described. Using fibrinogen as a model glycoprotein, we have selected boronic acid-modified DNA aptamers that have high affinities (low nM Kd) and the ability to recognize changes in the glycosylation site. The method developed should also be applicable to the development of aptamers for other glycoproducts, such as glycolipids and glycopeptides.Keywords
This publication has 17 references indexed in Scilit:
- Functional Aptamers and Aptazymes in Biotechnology, Diagnostics, and TherapyChemical Reviews, 2007
- The Aptamer HandbookPublished by Wiley ,2006
- Boronic AcidsPublished by Wiley ,2005
- Tuning the Specificity of a Synthetic Receptor Using a Selected Nucleic Acid ReceptorJournal of the American Chemical Society, 2004
- Boronic Acid-Based SensorsCurrent Organic Chemistry, 2002
- A detailed examination of boronic acid–diol complexationTetrahedron, 2002
- Artificial Receptors as Chemosensors for CarbohydratesPublished by Springer Science and Business Media LLC ,2001
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990
- Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNANature, 1990