Interleukin-17A and Neutrophils in a Murine Model of Bird-Related Hypersensitivity Pneumonitis
Open Access
- 14 September 2015
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 10 (9), e0137978
- https://doi.org/10.1371/journal.pone.0137978
Abstract
Hypersensitivity pneumonitis (HP) is an immune mediated lung disease induced by the repeated inhalation of a wide variety of antigens. Bird-related hypersensitivity pneumonitis (BRHP) is one of the most common forms of HP in human and results from the inhalation of avian antigens. The findings of a recent clinical analysis suggest that in addition to Th1 factors, the levels of interleukin(IL)-17 and IL-17-associated transcripts are increased in the setting of HP, and that both IL-17A and neutrophils are crucial for the development of pulmonary inflammation in murine models of HP. Our objectives were to investigate the roles of IL-17A and neutrophils in granuloma-forming inflammation in an acute HP model. We developed a mouse model of acute BRHP using pigeon dropping extract. We evaluated the process of granuloma formation and the roles of both IL-17A and neutrophils in a model. We found that the neutralization of IL-17A by the antibody attenuated granuloma formation and the recruitment of neutrophils, and also decreased the expression level of chemokine(C-X-C motif) ligand 5 (CXCL5) in the acute HP model. We confirmed that most of the neutrophils in the acute HP model exhibited immunoreactivity to the anti-IL-17 antibody. We have identified the central roles of both IL-17A and neutrophils in the pathogenesis of granuloma formation in acute HP. We have also assumed that neutrophils are an important source of IL-17A in an acute HP model, and that the IL-17A-CXCL5 pathway may be responsible for the recruitment of neutrophils.Keywords
This publication has 42 references indexed in Scilit:
- Chronic Hypersensitivity PneumonitisClinics in Chest Medicine, 2012
- Classification of Hypersensitivity PneumonitisInternational Archives of Allergy and Immunology, 2009
- Allergic alveolitis: new insights into old mysteriesRespiratory Medicine, 1993
- Hypersensitivity Pneumonitis in Athymic Nude Mice: Additional Evidence of T Cell DependencyAmerican Review of Respiratory Disease, 1992
- T Cells in Hypersensitivity Pneumonitis: Effects of In Vivo Depletion of T Cells in a Mouse ModelAmerican Journal of Respiratory Cell and Molecular Biology, 1992
- Murine Hypersensitivity Pneumonitis: A Study of Cellular Infiltrates and Cytokine Production and Its Modulation by Cyclosporin AAmerican Journal of Respiratory Cell and Molecular Biology, 1992
- Pigeon breeders’ lung: pigeon intestinal mucin, an antigen distinct from pigeon IgAClinical and Experimental Immunology, 1991
- Histologic Diagnosis of Extrinsic Allergic AlveolitisThe American Journal of Surgical Pathology, 1988
- Increase of HLA-DR7 in pigeon breeder's lung in a Mexican populationClinical Immunology and Immunopathology, 1987
- Hypersensitivity pneumonitisJournal of Allergy and Clinical Immunology, 1984