Hypersensitivity Pneumonitis in Athymic Nude Mice: Additional Evidence of T Cell Dependency
- 1 August 1992
- journal article
- research article
- Published by American Thoracic Society in American Review of Respiratory Disease
- Vol. 146 (2), 479-484
- https://doi.org/10.1164/ajrccm/146.2.479
Abstract
We previously demonstrated that C57 Black/6 mice develop lung lesions similar to human hypersensitivity pneumonitis (HP) by repeated transnasal administration of Thermoactinomyces vulgaris (Tv) antigen. To elucidate the role of T cells in the development of this disease, Tv antigen (90 µg/day) was transnasally administered to athymic nude C57 Black/nu/nu (nu) mice and their littermates (+/nu) three times a week for 3 wk. The nude mice developed minimal lung lesions, whereas their thymus-intact littermates (+/nu) showed changes equivalent to those in C57 Black/6. Changes in local inflammatory cell responses were evaluated by bronchoalveolar lavage, and increases in the numbers of lymphocytes and macrophages were significantly less severe in the nudemice than in the +/nu mice. Interestingly, the increase in polymorphonuclear leukocytes 6 h after the last antigen inoculation was equivalently seen in both groups. When spleen-derived T cells (more than 95% Thy-1.2+) from the sensitized +/nu mice were adoptively transferred to nude mice, the HP-like lesions in the recipients were found after Tv antigen challenge. These results suggest that Thy-1.2+ T cell-mediated immunity was necessary for the development of HP in this murine model.Keywords
This publication has 5 references indexed in Scilit:
- Experimental Hypersensitivity Pneumonitis in the Rat: Histopathology and T-cell Subset CompartmentalizationAmerican Journal of Respiratory Cell and Molecular Biology, 1991
- The inducing role of tumor necrosis factor in the development of bactericidal granulomas during BCG infectionCell, 1989
- Spontaneous Regression in Murine Hypersensitivity Pneumonitis: Lack of Immunological ToleranceInternational Archives of Allergy and Immunology, 1989
- Hypersensitivity pneumonitisJournal of Allergy and Clinical Immunology, 1987
- Experimental production of hypersensitivity pneumonitis with bagasse and thermophilic actinomycete antigenJournal of Allergy and Clinical Immunology, 1972