A New Method for Purifying Lambda DNA From Phage Lysates
- 1 February 1985
- journal article
- research article
- Published by Mary Ann Liebert Inc in DNA
- Vol. 4 (1), 39-49
- https://doi.org/10.1089/dna.1985.4.39
Abstract
A new method for preparing small quantities of .lambda. DNA from phage lysates was developed. The protocol is based on the concentration and purification of bacteriophage particles from crude lysates using small DEAE-cellulose columns. This chromatographic step gives an absolute separation of the .lambda. DNA from the cellular nuclei acids and a 20-fold enrichment relative to the major soluble proteins in crude lysates, while effecting a 10-fold concentration of the phage. Final deproteinization and concentration of the .lambda. DNA is achieved by conventional precipitation steps. The .lambda. DNA produced by this method is nondegraded, biologically active and an excellent substrate for restriction enzymes. A detailed protocol is provided for starting with individual plaques and using the method to obtain purified DNA from large numbers of .lambda. clones.This publication has 15 references indexed in Scilit:
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