Characterization of lamin B receptor of Sf9 cells and its fate during Autographa californica nucleopolyhedrovirus infection
- 3 April 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Cytotechnology
- Vol. 72 (2), 315-325
- https://doi.org/10.1007/s10616-020-00380-0
Abstract
Baculovirus nucleocapsids egress from the nuclear membrane during infection. However, details of alternation of nuclear membrane structure during baculovirus egress are unknown. In this study, we examined the changes of lamin B receptor (LBR), a main inner nuclear membrane component, during Autographa californica nucleopolyhedrovirus (AcMNPV) infection. Firstly, the open reading frame (Orf) of Sf9 lbr was cloned by reverse transcription PCR, and the distribution of LBR in Sf9 cells were observed by fusing LBR with the red fluorescence protein mcherry. Besides, the amount of endogenous LBR during AcMNPV infection was detected by western blotting. Moreover, the distribution of LBR after AcMNPV infection was observed under the confocal fluorescence microscopy. Furthermore, the effects of protein kinase C (PKC) inhibitor on stability of LBR and release of budded virus (BVs) were determined. The results showed that Sf9 lbr contains an Orf of 2040 nucleotides (NTs), which encodes a predicted protein of 679 amino acids (AAs). Fluorescence microscopy showed that LBR is localized to the nuclear membrane. Western blotting result showed that the amount of endogenous LBR is significantly reduced after AcMNPV infection. Transfection and infection assay demonstrated that the fluorescence of LBR nearly completely disappeared after viral infection. PKC inhibitor can suppress the degradation of LBR induced by AcMNPV, resulting in the reduction of viral titer of progeny viruses. The electron microscopy analysis demonstrated that PKC inhibitor did not influence virion entry, uncoating, and assembly, but may partially protect the nuclear membrane from disruption by AcMNPV. Taken together, AcMNPV infection can distort the expression of LBR, which may promote the egress of nucleocapsids.Keywords
Funding Information
- National Natural Science Foundation of China (31501701)
- Plant Foundation for Young Scientists of Henan University (CX0000A40557)
This publication has 31 references indexed in Scilit:
- Solution Structure and Molecular Interactions of Lamin B Receptor Tudor DomainJournal of Biological Chemistry, 2012
- Identification of Autographa californica Nucleopolyhedrovirus ac93 as a Core Gene and Its Requirement for Intranuclear Microvesicle Formation and Nuclear Egress of NucleocapsidsJournal of Virology, 2011
- Temporal control of nuclear envelope assembly by phosphorylation of lamin B receptorMolecular Biology of the Cell, 2011
- Disruption of Nuclear Organization during the Initial Phase of African Swine Fever Virus InfectionJournal of Virology, 2011
- Significance of host cell kinases in herpes simplex virus type 1 egress and lamin-associated protein disassembly from the nuclear laminaVirology, 2010
- Autographa californica Multiple Nucleopolyhedrovirus ac76 Is Involved in Intranuclear Microvesicle FormationJournal of Virology, 2010
- Epstein-Barr Virus BGLF4 Kinase Induces Disassembly of the Nuclear Lamina To Facilitate Virion ProductionJournal of Virology, 2008
- Autographa californica Multiple Nucleopolyhedrovirus EXON0 (ORF141) Is Required for Efficient Egress of Nucleocapsids from the NucleusJournal of Virology, 2007
- Herpes Simplex Virus Infection Induces Phosphorylation and Delocalization of Emerin, a Key Inner Nuclear Membrane ProteinJournal of Virology, 2007
- Cell Cycle Dynamics of the Nuclear EnvelopeThe Scientific World Journal, 2003