UDP‐GalNAc : GalNAc‐mucin α‐N‐acetylgalactosamine transferase activity in human intestinal cancerous tissues
- 14 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 190 (2), 259-262
- https://doi.org/10.1016/0014-5793(85)81295-3
Abstract
GalNAc transferase activities of 6 human intestinal cancerous tissues were examined using bovine submaxillary gland mucin and its desialylated derivative, asialomucin, as acceptors. A Triton X-100 extract of these tissues was used an an enzyme source. All the tissues examined had GalNAc transferase that catalyzes the transfer of GalNAc from UDP-GalNAc to serine or threonine residues of the polypeptide chain. One of 6 specimens showed in addition UDP-GalNAc:GalNAc-mucin alpha-GalNAc transferase activity, synthesizing a disaccharide unit, GalNAc alpha----GalNAc, when asialomucin was used as an acceptor. This carbohydrate structure was deduced on the basis of results of gel filtration, exoglycosidase digestion, and high-voltage paper electrophoresis.Keywords
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