Promotion of NEDD8-CUL1 Conjugate Cleavage by COP9 Signalosome
- 18 May 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 292 (5520), 1382-1385
- https://doi.org/10.1126/science.1059780
Abstract
SCF ubiquitin ligases control various processes by marking regulatory proteins for ubiquitin-dependent proteolysis. To illuminate how SCF complexes are regulated, we sought proteins that interact with the human SCF component CUL1. The COP9 signalosome (CSN), a suppressor of plant photomorphogenesis, associated with multiple cullins and promoted cleavage of the ubiquitin-like protein NEDD8 from Schizosaccharomyces pombe CUL1 in vivo and in vitro. Multiple NEDD8-modified proteins uniquely accumulated in CSN-deficient S. pombe cells. We propose that the broad spectrum of activities previously attributed to CSN subunits—including repression of photomorphogenesis, activation of JUN, and activation of p27 nuclear export—underscores the importance of dynamic cycles of NEDD8 attachment and removal in biological regulation.Keywords
This publication has 24 references indexed in Scilit:
- Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCF TIR1 in Mediating Auxin ResponseScience, 2001
- Covalent modifier NEDD8 is essential for SCF ubiquitin-ligase in fission yeastThe EMBO Journal, 2000
- p38 Binds to the Intracellular Precursor of the Lutropin/Choriogonadotropin Receptor and Promotes Its DegradationOnline Journal of Public Health Informatics, 2000
- Unified nomenclature for the COP9 signalosome and its subunits: an essential regulator of developmentTrends in Genetics, 2000
- Modification of Cullin-1 by Ubiquitin-like Protein Nedd8 Enhances the Activity of SCFskp2 toward p27kip1Biochemical and Biophysical Research Communications, 2000
- MALDI Quadrupole Time-of-Flight Mass Spectrometry: A Powerful Tool for Proteomic ResearchAnalytical Chemistry, 2000
- The COP9/signalosome complex is conserved in fission yeast and has a role in S phaseCurrent Biology, 1999
- SCF and Cullin/RING H2-Based Ubiquitin LigasesAnnual Review of Cell and Developmental Biology, 1999
- Characterization and Purification of the Mammalian COP9 Complex, a Conserved Nuclear Regulator Initially Identified as a Repressor of Photomorphogenesis in Higher PlantsPhotochemistry and Photobiology, 1998
- A new group of conserved coactivators that increase the specificity of AP-1 transcription factorsNature, 1996