Characterizing and Mapping Porcine Endogenous Retroviruses in Westran Pigs
- 1 June 2002
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 76 (11), 5548-5556
- https://doi.org/10.1128/jvi.76.11.5548-5556.2002
Abstract
Since porcine endogenous retroviruses (PERVs) can infect cultured human cells, they are a potential hazard to xenotransplantation. For this reason, endogenous retroviruses from the Westran (Westmead Hospital transplantation) inbred line of pigs were analyzed by using consensus primers for the type A and type B viruses to amplify 1.8-kb envelope gene fragments. After preliminary analysis with restriction enzymes Kpn I and Mbo I, 31 clones were sequenced. Between types A and B, five recombinant clones were identified. Fifty-five percent of clones (17 of 31) had premature stop codons within the envelope protein-encoding region. Endogenous retroviruses in Westran pigs were physically mapped by fluorescence in situ hybridization (FISH) using PERV-A and PERV-B envelope clones as probes to identify at least 32 integration sites (19 PERV-A sites and 13 PERV-B sites). The chromosomal sites of integration in the Westran strain are quite different from those in the European Large White pig. The recombinant clones suggest that defective PERVs could become infective through recombination and further that PERVs might recombine with human endogenous retroviruses in xenotransplants.Keywords
This publication has 69 references indexed in Scilit:
- Porcine endogenous retrovirus (PERV) was not transmitted from transplanted porcine endothelial cells to baboons in vivoTransplant International, 1998
- Porcine endogenous retrovirus (PERV) was not transmitted from transplanted porcine endothelial cells to baboons in vivoTransplant International, 1998
- Assignment of the pyruvate carboxylase gene to rat chromosome band 1q43 by in situ hybridizationCytogenetic and Genome Research, 1997
- Statistical analysis of in situ hybridization data: Derivation and use of the Zmax testGenomics, 1992
- Molecular cloning of unintegrated closed circular DNA of porcine retrovirusFEBS Letters, 1986
- Some characteristics of a porcine retrovirus from a cell line derived from swine malignant lymphomasFEBS Letters, 1985
- THE KANGAROO ISLAND STRAIN OF PIG IN BIOMEDICAL RESEARCHAustralian Veterinary Journal, 1981
- Genetic Recombination in Rous Sarcoma Virus: the Genesis of Recombinants and Lack of Evidence for Linkage between pol, env and src Genes in Three Factor CrossesJournal of General Virology, 1979
- Evidence for genetic recombination between endogenous and exogenous mouse RNA type C virusesCell, 1974
- Virus-like particles occurring in cultures of stable pig kidney cell linesArchiv für die gesamte Virusforschung, 1970