Influence of Seasons, Extenders, Slow and Rapid Freezing on Seminal Characters in Korean Native Bucks
- 12 January 2006
- journal article
- research article
- Published by Wiley in Reproduction in Domestic Animals
- Vol. 41 (1), 55-60
- https://doi.org/10.1111/j.1439-0531.2006.00652.x
Abstract
The success of AI technology is based on both semen quality and freezing process. In order to establish the semen freezing techniques in Korean native bucks, factors affecting the success were evaluated in the present study. Semen collected by electro-ejaculation from bucks during four distinct seasons was evaluated for semen volume and pH, sperm motility and survivability. The semen volume, concentration and total cell were higher in spring, summer and less in winter. Yet, there were no seasonal differences in the proportional data of live sperm, motility score and pH of semen among seasons. The percentage of live sperm after thawing was found to increase with increased concentration of lactose in Tris-Egg yolk-glycerol (TY-G), being highest in TY-G supplemented with 180 mm lactose (TYL180-G), but did not differ between TY-G and TYL120-G. Sperm motility was enhanced by employing 2.0 h equilibration time with rapid freezing method. In conclusion, semen could be frozen with high success rates for further use of AI in breeding techniques and to preserve the Korean native bucks.Keywords
This publication has 25 references indexed in Scilit:
- The Effect of Removal of Seminal Plasma, Egg Yolk Level and Season on Sperm Freezability of Canary Buck (Capra hircus)Reproduction in Domestic Animals, 2005
- Successful pregnancies with directional freezing of large volume buck semenTheriogenology, 2005
- Cloning and seasonal secretion of the pancreatic lipase-related protein 2 present in goat seminal plasmaBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2005
- Characteristics and seasonal variations in the semen of Alpine, Saanen and Damascus goat bucks born and raised in GreeceTheriogenology, 2000
- Viability Assessment of Mammalian Sperm Using SYBR-14 and Propidium Iodide1Biology of Reproduction, 1995
- Effect of glycerolization procedure and removal of seminal plasma on post-thaw survival and got-release from Boer goat spermatozoaTheriogenology, 1994
- Studies of chemical components of Angora goat seminal plasmaTheriogenology, 1989
- Effect of glycerol level in Tris-basek extender and equilibration period on quality of frozen goat semenTheriogenology, 1986
- VIABILITÉ DES SPERMATOZOÏDES DE BOUC CONSERVÉS ET CONGELÉS AVEC OU SANS LEUR PLASMA SÉMINAL : EFFET DU GLUCOSEAnnales de Biologie Animale Biochimie Biophysique, 1974
- Survival of Spermatozoa at Low TemperaturesNature, 1950