Reversible thermal unfolding ofBacillus subtilislevansucrase is modulated by Fe3+and Ca2+
- 26 November 1990
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 275 (1-2), 61-64
- https://doi.org/10.1016/0014-5793(90)81439-u
Abstract
The equilibrium transition curves for thermal unfolding of levansucrase were established at several pH values. At pH 7 and within the temperature range of bacterial growth, the unfolded from is predominant. However, under such conditions, refolding is promoted by the only addition of Ca2+ or Fe3+. We propose that the tertiary structure flexibility of levansucrase plays a key role in its secretion process.Keywords
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