Simultaneous Determination of Human Enterovirus 71 and Coxsackievirus B3 by Dual-Color Quantum Dots and Homogeneous Immunoassay
- 5 March 2012
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 84 (7), 3200-3207
- https://doi.org/10.1021/ac203172x
Abstract
Human Enterovirus 71 (EV71) and Coxsackievirus B3 (CVB3) have high risks for morbidity and mortality. A virus quantitation immunoassay has been proposed by employing two colored quantum dots (QDs), antibodies of the virus, and graphene oxide (GO). The QDs are streptavidin-conjugated quantum dots (SA-QDs), and the antibodies are biotinylated antibodies. Biotinylated EV71 antibody (Ab1) was associated with 525 nm green colored SA-QDs via biotin-streptavidin interaction forming QDs-Ab1, whereas biotinylated CVB3 antibody (Ab2) was associated with 605 nm red colored SA-QDs via biotin–streptavidin interaction forming QDs-Ab2. GO was an excellent quencher to the fluorescence of both QDs-Ab1 and QDs-Ab2. The targets of EV71 and CVB3 can break up the complex of QDs-Ab and GO, recovering the fluorescence of QDs-Ab1 and QDs-Ab2, respectively. Using these two different colored QDs-Ab fluorescence recovery intensities upon the addition of targets EV71 and CVB3, the two enteroviruses can be simultaneously quantitatively determined with a single excitation light. The detection limits of EV71 and CVB3 are 0.42 and 0.39 ng mL–1 based on 3 times signal-to-noise ratio, respectively. More importantly, this strategy can be further used as a universal method for any protein or virus determination by changing the conjugated antibodies in disease early diagnosis, which can provide a fast and promising clinical approach for virus differentiation and determination. In a word, a simple, fast, sensitive, and highly selective assay for EV71 and CVB3 has been developed. It could be applied in clinical sample analysis with a satisfactory result. It was notable that the sensor could not only achieve rapid and precise quantitative determination of protein/virus by fluorescent intensity but also could be applied in semiquantitative protein/virus determination by digital visualization.Keywords
This publication has 37 references indexed in Scilit:
- Cultivation of Chlorella pyrenoidosa in soybean processing wastewaterBioresource Technology, 2011
- Optimization of nanowire DNA sensor sensitivity using self-consistent simulationNanotechnology, 2011
- Transfer-Printing of Single DNA Molecule Arrays on Graphene for High-Resolution Electron Imaging and AnalysisNano Letters, 2011
- Functionalization and Self-Assembly of DNA Bidimensional ArraysInternational Journal of Molecular Sciences, 2011
- Crystalline Two‐Dimensional DNA‐Origami ArraysAngewandte Chemie, 2010
- EV71: An emerging infectious disease vaccine target in the Far East?Vaccine, 2010
- CdTe Quantum Dots (QDs) Based Kinetic Discrimination of Fe2+ and Fe3+, and CdTe QDs-Fenton Hybrid System for Sensitive Photoluminescent Detection of Fe2+Analytical Chemistry, 2009
- Epidemiology of EV71 Outbreaks in the Region in the Past DecadeInternational Journal of Infectious Diseases, 2008
- Identification of immunodominant VP1 linear epitope of enterovirus 71 (EV71) using synthetic peptides for detecting human anti-EV71 IgG antibodies in western blotsClinical Microbiology & Infection, 2008
- Adenovirus in EV71-associated hand, foot, and mouth diseaseThe Lancet, 2000