Design of DNA-Binding Peptides Based on the Leucine Zipper Motif
- 17 August 1990
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 249 (4970), 774-778
- https://doi.org/10.1126/science.2389143
Abstract
A class of transcriptional regulator proteins bind to DNA at dyad-symmetric sites through a motif consisting of (i) a "leucine zipper" sequence that associates into noncovalent, parallel, alpha-helical dimers and (ii) a covalently connected basic region necessary for binding DNA. The basic regions are predicted to be disordered in the absence of DNA and to form alpha helices when bound to DNA. These helices bind in the major groove forming multiple hydrogen-bonded and van der Waals contacts with the nucleotide bases. To test this model, two peptides were designed that were identical to natural leucine zipper proteins only at positions hypothesized to be critical for dimerization and DNA recognition. The peptides form dimers that bind specifically to DNA with their basic regions in alpha-helical conformations.Keywords
This publication has 41 references indexed in Scilit:
- Biochemical analysis of transcriptional activation by Jun: Differential activity of c- and v-JunCell, 1989
- An alpha-helical peptide model for electrostatic interactions of proteins with DNA: The N terminus of RecAJournal of Molecular Biology, 1989
- A Protein That Binds to a cis-acting Element of Wheat Histone Genes Has a Leucine Zipper MotifScience, 1989
- A Fos protein containing the Jun leucine zipper forms a homodimer which binds to the AP1 binding siteNature, 1989
- Hydrophobic Organization of Membrane ProteinsScience, 1989
- Analysis of the relationship between side-chain conformation and secondary structure in globular proteinsJournal of Molecular Biology, 1987
- Structure of ferricytochrome c′ from Rhodospirillum molischianum at 1.67 Å resolutionJournal of Molecular Biology, 1985
- Determination of the helix and β form of proteins in aqueous solution by circular dichroismBiochemistry, 1974
- Optimised parameters for A-DNA and B-DNABiochemical and Biophysical Research Communications, 1972
- Computed circular dichroism spectra for the evaluation of protein conformationBiochemistry, 1969