Alteration of Fermentation in Continuous Culture of Mixed Rumen Bacteria by Isolated Alfalfa Saponins
Open Access
- 1 April 1987
- journal article
- Published by American Dairy Science Association in Journal of Dairy Science
- Vol. 70 (4), 799-805
- https://doi.org/10.3168/jds.s0022-0302(87)80076-0
Abstract
Saponins isolated from alfalfa by ethanol extraction and acid hydrolysis were incorporated into protein-free purified diets at 0, .5, 1, 2, and 4% of dietary dry matter to investigate the effect of saponins on fermentation by mixed rumen bacteria maintained in continuous culture. Inoculum was obtained from a lactating dairy cow fed alfalfa hay and a 13% crude protein grain mixture with forage to grain ratios of 33:67, 67:33, or 100:0. Outflow of microbial protein was lower in fermenters fed purified diet containing 1% saponins. Accumulation of ammonia was observed in fermenters fed purified diets containing alfalfa saponins. Total volatile fatty acid production was reduced by addition of isolated alfalfa saponin fraction at .5, 1, 2, and 4% concentrations. Acetate to propionate ratios were reduced from 1.93 in control to 1.37 in fermenters with 1% saponins. Extent of change in fermentation was not proportional to the concentration of added saponins.Keywords
This publication has 25 references indexed in Scilit:
- Saponin content of food plants and some prepared foodsJournal of the Science of Food and Agriculture, 1983
- Protein quality of high and low saponin alfalfa protein concentrateJournal of the Science of Food and Agriculture, 1980
- Distribution of saponin in alfalfa protein recovery systemsJournal of Agricultural and Food Chemistry, 1979
- Effect of alfalfa saponins on the growth and some aspects of lipid metabolism of mice and quailsJournal of the Science of Food and Agriculture, 1976
- Inhibitory Effects of Perloline to Rumen Fermentation in vitro1Crop Science, 1972
- Lucerne saponins II.—Purification and fractionation of saponins from lucerne tops and roots and characterisation of the isolated fractionsJournal of the Science of Food and Agriculture, 1970
- Steam distillation methods for determination of ammonium, nitrate and nitriteAnalytica Chimica Acta, 1965
- Action of Saponin on Biological Cell MembranesNature, 1962
- Dissimilation of Alfalfa Saponins by Rumen BacteriaScience, 1958
- Oxidation-Reduction Aspects of ResazurinNature, 1945