Identification of Tsetse (Glossina spp.) Using Matrix-Assisted Laser Desorption/Ionisation Time of Flight Mass Spectrometry
Open Access
- 11 July 2013
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLoS Neglected Tropical Diseases
- Vol. 7 (7), e2305
- https://doi.org/10.1371/journal.pntd.0002305
Abstract
Glossina (G.) spp. (Diptera: Glossinidae), known as tsetse flies, are vectors of African trypanosomes that cause sleeping sickness in humans and nagana in domestic livestock. Knowledge on tsetse distribution and accurate species identification help identify potential vector intervention sites. Morphological species identification of tsetse is challenging and sometimes not accurate. The matrix-assisted laser desorption/ionisation time of flight mass spectrometry (MALDI TOF MS) technique, already standardised for microbial identification, could become a standard method for tsetse fly diagnostics. Therefore, a unique spectra reference database was created for five lab-reared species of riverine-, savannah- and forest- type tsetse flies and incorporated with the commercial Biotyper 3.0 database. The standard formic acid/acetonitrile extraction of male and female whole insects and their body parts (head, thorax, abdomen, wings and legs) was used to obtain the flies' proteins. The computed composite correlation index and cluster analysis revealed the suitability of any tsetse body part for a rapid taxonomical identification. Phyloproteomic analysis revealed that the peak patterns of G. brevipalpis differed greatly from the other tsetse. This outcome was comparable to previous theories that they might be considered as a sister group to other tsetse spp. Freshly extracted samples were found to be matched at the species level. However, sex differentiation proved to be less reliable. Similarly processed samples of the common house fly Musca domestica (Diptera: Muscidae; strain: Lei) did not yield any match with the tsetse reference database. The inclusion of additional strains of morphologically defined wild caught flies of known origin and the availability of large-scale mass spectrometry data could facilitate rapid tsetse species identification in the future. Tsetse flies are confined to tropical Africa and are carriers for trypanosomes, single-celled blood parasites. Through the bite of an infective tsetse, people and animals may contract trypanosomiasis, a degenerative disease leading to death if left untreated. Tsetse control proved effective for disease containment, but data on the flies as tsetse identification are a prerequisite for planning any control intervention. There are 32 generally accepted tsetse species and subspecies. Classical species identification relies on minor morphological differences, often challenging for field workers. In the last decade, Matrix-Assisted Laser Desorption/Ionisation (MALDI) has revolutionised microbial species identification. After a simple protein extraction, a laser-induced ionisation takes place. Then, the ions are accelerated in a vacuum tube, and their Time of Flight (ToF) to reach the detector is recorded. The protein composition of each organism is unique, and so is their MALDI signature. Comparison of the obtained signature with a database of known organisms enables rapid identification as reliable as genome-based methods. To possibly speed up tsetse diagnostics, we established a MALDI database for the identification of five defined laboratory tsetse breeds. Inclusion of wild-caught tsetse could reinforce the reference database for the identification of tsetse at the species and subspecies level.Keywords
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