Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges
- 1 December 2010
- journal article
- research article
- Published by Wiley in Medical and Veterinary Entomology
- Vol. 25 (1), 32-38
- https://doi.org/10.1111/j.1365-2915.2010.00927.x
Abstract
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has shown promise in species identification of insect species. We evaluated its potential to consistently characterize laboratory-reared biting midges of the species Culicoides nubeculosus (Meigen) (Diptera: Ceratopogonidae). Twenty-one reproducible potential biomarker masses for C. nubeculosus were identified under different experimental treatments. These treatments included the homogenization of insects in either water or known concentrations of formic acid. The biomarker masses were present independent of age, gender and different periods of storage of individuals in 70% ethanol (a standard preservation method). It was found that the presence of blood in females reduced the intensity of the MALDI-TOF pattern, necessitating the removal of the abdomen before analysis. The protein profiles of a related non-biting midge, Forcipomyia sp. (Diptera: Ceratopogonidae), and of Aedes japonicus japonicus (Theobald) (Diptera: Culicidae) mosquitoes were also examined and were distinctly different. These findings provide preliminary data to optimize future studies in differentiation of species within the Culicoides genus using MALDI-TOF MS which is a rapid, simple, reliable and cost-effective technique.Keywords
This publication has 20 references indexed in Scilit:
- Discrimination of different species from the genus Drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometryBMC Evolutionary Biology, 2010
- Discrimination ofCulicoidesMidge Larvae Using Multiplex Polymerase Chain Reaction Assays Based on DNA Sequence Variation at the Mitochondrial Cytochrome C Oxidase I GeneJournal of Medical Entomology, 2009
- PCR identification of Culicoides dewulfi midges (Diptera: Ceratopogonidae), potential vectors of bluetongue in GermanyZeitschrift für Parasitenkunde, 2009
- Laboratory Colonization of Aedes japonicus japonicusJournal of the American Mosquito Control Association, 2008
- Fish Authentication by MALDI-TOF Mass SpectrometryJournal of Agricultural and Food Chemistry, 2008
- Development and evaluation of a real-time quantitative PCR assay for Culicoides imicola, one of the main vectors of bluetongue (BT) and African horse sickness (AHS) in Africa and EuropeResearch in Veterinary Science, 2008
- Control techniques for Culicoides biting midges and their application in the U.K. and northwestern PalaearcticMedical and Veterinary Entomology, 2008
- Molecular Identification of Western European Species of Obsoletus Complex (Diptera: Ceratopogonidae) by an Internal Transcribed Spacer-1 rDNA Multiplex Polymerase Chain Reaction AssayJournal of Medical Entomology, 2007
- Characterization of intact microorganisms by MALDI mass spectrometryMass Spectrometry Reviews, 2001
- Culicoides Biting Midges: Their Role as Arbovirus VectorsAnnual Review of Entomology, 2000