Noncoding Effects of Circular RNA CCDC66 Promote Colon Cancer Growth and Metastasis
Top Cited Papers
Open Access
- 30 April 2017
- journal article
- research article
- Published by American Association for Cancer Research (AACR) in Cancer Research
- Vol. 77 (9), 2339-2350
- https://doi.org/10.1158/0008-5472.can-16-1883
Abstract
Circular RNA (circRNA) is a class of noncoding RNA whose functions remain mostly unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but direct evidence is scarce. Here, we characterize the functional role of a novel circRNA, circCCDC66, in colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated in cancer cells, several of which were verified by quantitative RT-PCR. CircCCDC66 expression was elevated in polyps and colon cancer and was associated with poor prognosis. Gain-of-function and loss-of-function studies in colorectal cancer cell lines demonstrated that circCCDC66 controlled multiple pathological processes, including cell proliferation, migration, invasion, and anchorage-independent growth. In-depth characterization revealed that circCCDC66 exerts its function via regulation of a subset of oncogenes, and knockdown of circCCDC66 inhibited tumor growth and cancer invasion in xenograft and orthotopic mouse models, respectively. Taken together, these findings highlight a novel oncogenic function of circRNA in cancer progression and metastasis. Cancer Res; 77(9); 2339–50. ©2017 AACR.Other Versions
Funding Information
- Ministry of Science and Technology of Taiwan (104-2320-B-006-036-MY3)
- National Health Research Institute (NHRI-EX102-10244BI)
- National Cheng Kung University (D103-35A17)
- Cancer Prevention Research Institute of Texas (RP160283)
- National Institutes of Health (R01EB013584)
This publication has 38 references indexed in Scilit:
- Circular RNA profiling reveals an abundant circHIPK3 that regulates cell growth by sponging multiple miRNAsNature Communications, 2016
- Hsa_circ_0001649: A circular RNA and potential novel biomarker for hepatocellular carcinomaCancer Biomarkers, 2016
- Decreased expression of hsa_circ_001988 in colorectal cancer and its clinical significances.2015
- Combinatorial control of Drosophila circular RNA expression by intronic repeats, hnRNPs, and SR proteinsGenes & Development, 2015
- Circular RNA biogenesis can proceed through an exon-containing lariat precursoreLife, 2015
- Using circular RNA as a novel type of biomarker in the screening of gastric cancerClinica Chimica Acta; International Journal of Clinical Chemistry, 2015
- CIRI: an efficient and unbiased algorithm for de novo circular RNA identificationGenome Biology, 2015
- MicroRNA-146a directs the symmetric division of Snail-dominant colorectal cancer stem cellsNature, 2014
- Circular RNAs are a large class of animal RNAs with regulatory potencyNature, 2013
- Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profilesProceedings of the National Academy of Sciences of the United States of America, 2005