Decreased expression of hsa_circ_001988 in colorectal cancer and its clinical significances.
- 1 December 2015
- journal article
- Vol. 8 (12), 16020-5
Abstract
Circular RNA (circRNA) is a type of RNAs which, unlike the better known linear RNA, forms a covalently closed continuous loop. They have emerged recently as a new player in governing fundamental biological processes. However it remains elusive about the correlation of hsa_circ_001988 abundance with colorectal cancer. To investigate the circular RNA expression in colorectal cancer, the targeted hsa_circ_001988 was selected from next generation sequence data base generated in house and then designed divergent primers to amplify hsa_circ_001988 and sequenced it for validation. The expression of hsa_circ_001988 in 31 matched colorectal cancer tissue and normal colon mucosa was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). We used ΔCt method and investigated the differences between tumor tissues and normal colon mucosa by paired t-test. One-way analysis of variance was conducted to analyze the relationship between hsa_circ_001988 expression level and clinic pathological factors of patients. Receiver operating characteristic (ROC) curve was built by SPSS to evaluate the diagnostic values. The expression of hsa_circ_001988 was significantly correlated with differentiation (P<0.05) and perineural invasion (P<0.05). The area under ROC curve of hsa_circ_001988 was 0.788 (P<0.05). Those results indicate that hsa_circ_001988 may become a novel potential biomarker in the diagnosis of colorectal cancer and a potential novel target for the treatment of colorectal cancer.Keywords
This publication has 19 references indexed in Scilit:
- Using circular RNA as a novel type of biomarker in the screening of gastric cancerClinica Chimica Acta; International Journal of Clinical Chemistry, 2015
- Circles within circles: commentary on Ghosal et al. (2013) “Circ2Traits: a comprehensive database for circular RNA potentially associated with disease and traitsâ€Frontiers in Genetics, 2015
- Circular Intronic Long Noncoding RNAsMolecular Cell, 2013
- Circular RNAs are a large class of animal RNAs with regulatory potencyNature, 2013
- Transcriptome-wide discovery of circular RNAs in ArchaeaNucleic Acids Research, 2011
- Expression of Linear and Novel Circular Forms of an INK4/ARF-Associated Non-Coding RNA Correlates with Atherosclerosis RiskPLoS Genetics, 2010
- Perineural invasion in pT3N0 rectal cancerCancer, 2010
- The ubiquitous nature of cancer: the role of the SCFFbw7 complex in development and transformationOncogene, 2010
- Loss of FBXW7, a cell cycle regulating gene, in colorectal cancer: Clinical significanceInternational Journal of Cancer, 2009
- Scrambled exonsCell, 1991