Differentiation of Infectious Laryngotracheitis Virus Isolates by Restriction Fragment Length Polymorphic Analysis of Polymerase Chain Reaction Products Amplified from Multiple Genes
- 1 March 2006
- journal article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 50 (1), 28-33
- https://doi.org/10.1637/7414-072205r.1
Abstract
Infectious laryngotracheitis (ILT) has been identified in most countries around the world and remains a threat to the intensive poultry industry. Outbreaks of mild to moderate forms of ILT are common in commercial layer flocks, while sporadic outbreaks of ILT in broiler flocks have also been recognized as an emerging problem in several countries. Examination of viral isolates using restriction fragment length polymorphism of polymerase chain reaction (PCR-RFLP) from individual ILTV genes has suggested that some of these outbreaks were caused by vaccine strains. In this study, PCR-RFLP of a number of ILTV genes/genomic regions including gE, gG, TK, ICP4, ICP18.5, and open reading frame (ORF) B-TK was used to examine a number of historical and contemporary Australian ILTV isolates and vaccine strains. PCR-RFLP of gE using restriction endonuclease EaeI failed to distinguish between any of the isolates including the vaccine strains. PCR-RFLP of gG, TK, and ORFB-TK using restriction endonucleases MspI and FokI, respectively, divided all the isolates into two groups. PCR-RFLP of ICP18.5 and ICP4 using restriction endonuclease HaeIII separated the isolates into three different groups with some field isolates only able to be distinguished from vaccine strains by PCR-RFLP of ICP18.5. A combination of groupings including gG, TK, ICP4, ICP18.5, and ORFB-TK PCR-RFLP classified the ILTV isolates under investigation into five different groups with most isolates distinguishable from vaccine strains. Results from this study reveal that to achieve reliable identification of strains of ILTV, the examination of multiple gene regions will be required, and that most of the recent ILT outbreaks in Australia are not being caused by vaccine strains.Keywords
This publication has 14 references indexed in Scilit:
- Efficacy of Live Virus Vaccines Against Infectious Laryngotracheitis Assessed by Polymerase Chain Reaction–Restriction Fragment Length PolymorphismAvian Diseases, 2003
- Comparison of virulence and restriction endonuclease cleavage patterns of infectious laryngotracheitis viruses isolated in KoreaAvian Pathology, 2001
- RFLP analysis of recent Northern Ireland isolates of infectious laryngotracheitis virus: Comparison with vaccine virus and field isolates from England, Scotland and the Republic of IrelandAvian Pathology, 2000
- Differential sensitivity of culture and the polymerase chain reaction for detection of feline herpesvirus 1 in vaccinated and unvaccinated catsArchiv für die gesamte Virusforschung, 1997
- Gallid herpesvirus 1 (infectious laryngotracheitis virus): cloning and physical maps of the SA-2 strainArchiv für die gesamte Virusforschung, 1991
- Virulence of five live vaccines against Avian Infectious Laryngotracheitis and their immunogenicity and spread after eyedrop or spray applicationVeterinary Quarterly, 1987
- Laryngotracheitis herpesvirus infection in the chicken: The role of humoral antibody in immunity to a graded challenge infectionAvian Pathology, 1983
- Mathematical model for studying genetic variation in terms of restriction endonucleases.Proceedings of the National Academy of Sciences of the United States of America, 1979
- AEROSOL ADMINISTRATION OF THE SA‐2 VACCINE STRAIN OF INFECTIOUS LERYNGOTRACHEITIS VIRUSAustralian Veterinary Journal, 1974
- The development of a live attenuated infectious laryngotracheitis vaccineVeterinary Record, 1965