Efficacy of Live Virus Vaccines Against Infectious Laryngotracheitis Assessed by Polymerase Chain Reaction–Restriction Fragment Length Polymorphism
- 1 April 2003
- journal article
- research article
- Published by American Association of Avian Pathologists (AAAP) in Avian Diseases
- Vol. 47 (2), 261-271
- https://doi.org/10.1637/0005-2086(2003)047[0261:eolvva]2.0.co;2
Abstract
The efficacy of four different commercial live vaccines (vaccines A, B, C, and D) against the infectious laryngotracheitis virus (ILTV) was assessed in specific-pathogen-free (SPF) chickens. SPF chickens were vaccinated intraocularly at 6 wk old with ILTV live vaccines and were challenged intratracheally with the N91B01 strain of virulent Korean ILTV 2 wk after vaccination. The immunity against ILTV live vaccines was assessed by the incidence of latent infection by the challenge virus in the chickens' tracheas and trigeminal ganglia, the reisolation rate of the challenge virus, and the clinical signs in the chickens challenged with the N91B01 strain of ILTV. The latent infection in chickens was assessed by nested polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP). Our data showed that the clinical signs and challenge virus isolation were negative in all chickens receiving four different commercial ILTV live vaccines. The viral DNA of the vaccine strain, but not that of the challenge virus, was detected in chickens vaccinated with vaccine A by nested PCR-RFLP. The viral DNAs of both the vaccine and challenge strains were detected from chickens vaccinated with vaccines B, C, and D. This study showed that only vaccine A can protect chickens from latent infection with the field virulent ILTV. We speculate that the efficacy of infectious laryngotracheitis live vaccines to protect chickens from latent infection with virulent ILTVs can be assessed by nested PCR-RFLP analysis.Keywords
This publication has 25 references indexed in Scilit:
- Comparison of virulence and restriction endonuclease cleavage patterns of infectious laryngotracheitis viruses isolated in KoreaAvian Pathology, 2001
- Demonstration of sites of latency of infectious laryngotracheitis virus using the polymerase chain reactionJournal of General Virology, 1992
- Humoral and cell-mediated immune responses to the glycoproteins of infectious laryngotracheitis herpesvirusArchiv für die gesamte Virusforschung, 1990
- Analysis of the nucleotide sequence of DNA from the region of the thymidine kinase gene of infectious laryngotracheitis virus; potential evolutionary relationships between the herpesvirus subfamiliesJournal of General Virology, 1990
- Detection of pseudorabies virus DNA sequences by the polymerase chain reactionArchiv für die gesamte Virusforschung, 1989
- Laryngotracheitis (gallid‐1) herpesvirus infection in the chicken 4. latency establishment by wild and vaccine strains of ILT virusAvian Pathology, 1986
- Experimental reactivation of equid herpesvirus 1 (EHV 1) following the administration of corticosteroidsEquine Veterinary Journal, 1985
- Herpesvirus hominis : Isolation from Human Trigeminal GanglionScience, 1972
- PERSISTENCE OF VIRUS IN RESPIRATORY INFECTIONS OF CHICKENSAustralian Veterinary Journal, 1972
- Cell Culture Techniques for Primary Isolation of Marek's Disease-Associated HerpesvirusAvian Diseases, 1969