Cloning, expression and modulation of a mouse NMDA receptor subunit
- 23 March 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 300 (1), 39-45
- https://doi.org/10.1016/0014-5793(92)80160-i
Abstract
The primary structure and presence of two forms of the mouse N-methyl-D-aspartate (NMDA) receptor channel subunit zeta 1 have been disclosed by cloning and sequencing the cDNAs. The zeta 1 subunit shows approximately 20% amino acid sequence identities with the rodent alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)- or kainate-selective GluR subunits and has structural features common to neurotransmitter-gated ion channels. Functional homomeric zeta 1 channels expressed in Xenopus oocytes by injection of the subunit-specific mRNA exhibit current responses characteristic for the NMDA receptor channel such as activation by glycine, Ca2+ permeability, blocking by Mg2+ and activation by polyamine. It has been found that the zeta 1 channel activity is positively modulated by treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA).Keywords
This publication has 36 references indexed in Scilit:
- A single amino acid residue determines the Ca2+ permeability of AMPA-selective glutamate receptor channelsBiochemical and Biophysical Research Communications, 1991
- A ring of uncharged polar amino acids as a component of channel constriction in the nicotinic acetylcholine receptorFEBS Letters, 1991
- Sustained potentiation of NMDA receptor-mediated glutamate responses through activation of protein kinase C by a μ opioidNeuron, 1991
- Functional expression from cloned cDNAs of glutamate receptor species responsive to kainate and quisqualateFEBS Letters, 1990
- A Family of AMPA-Selective Glutamate ReceptorsScience, 1990
- The Excitatory Amino Acid Receptors: Their Classes, Pharmacology, and Distinct Properties in the Function of the Central Nervous SystemAnnual Review of Pharmacology and Toxicology, 1989
- NMDA receptors - their role in long-term potentiationTrends in Neurosciences, 1987
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- Magnesium gates glutamate-activated channels in mouse central neuronesNature, 1984
- 2-Amino-5-phosphonovalerate (2APV), a potent and selective antagonist of amino acid-induced and synaptic excitationNeuroscience Letters, 1981