Lipidic phase membrane protein serial femtosecond crystallography
- 29 January 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Methods
- Vol. 9 (3), 263-265
- https://doi.org/10.1038/nmeth.1867
Abstract
Lipidic sponge phase crystallization yields membrane protein microcrystals that can be injected into an X-ray free electron laser beam, yielding diffraction patterns that can be processed to recover the crystal structure. X-ray free electron laser (X-FEL)-based serial femtosecond crystallography is an emerging method with potential to rapidly advance the challenging field of membrane protein structural biology. Here we recorded interpretable diffraction data from micrometer-sized lipidic sponge phase crystals of the Blastochloris viridis photosynthetic reaction center delivered into an X-FEL beam using a sponge phase micro-jet.This publication has 21 references indexed in Scilit:
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