Transcription of Ti plasmid-derived sequences in three octopine-type crown gall tumor lines.
- 1 June 1979
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 76 (6), 2828-2832
- https://doi.org/10.1073/pnas.76.6.2828
Abstract
Total RNA isolated from three octopine-type crown gall lines contains sequences homologous to specific regions of the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens strain 15955. A comparison of transcripts in these three tumor lines suggests that tumor cells transcribe various sequences within a sector of plasmid DNA of 13 x 10(6) daltons and that transcription may not be uniform across the plasmid derived sequences (T-DNA). Transcription of T-DNA by octopine-type tumors occurs at four major sites. The levels of transcription occurring at three of these sites appear to vary considerably among the three tumor lines investigated. Part of this variability may reflect differences in the organization and copy number of T-DNA. One of the transcription sites maps within a region of DNA with common sequence homology with all Ti plasmids. Varying amounts of transcript homologous to this region of T-DNA are present in all three tumor lines. It is suggested that transcription of these conserved sequences in the plant may have significance regarding the mechanism of tumorigenesis.This publication has 17 references indexed in Scilit:
- Restriction endonuclease mapping of a plasmid that confers oncogenicity upon Agrobacterium tumefaciens strain B6-806Plasmid, 1978
- Fingerprints of Agrobacterium Ti plasmidsPlasmid, 1978
- Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesisCell, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Charon Phages: Safer Derivatives of Bacteriophage Lambda for DNA CloningScience, 1977
- Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gelsJournal of Molecular Biology, 1977
- Octopine as a marker for the induction of tumorous growth by agrobacterium tumefaciens strain B6Biochemical and Biophysical Research Communications, 1976
- On the isolation of TI-plasmid from Agrobacterium tumefaciens.Nucleic Acids Research, 1976
- Octopine and nopaline synthesis and breakdown genetically controlled by a plasmid of Agrobacterium tumefaciensMolecular Genetics and Genomics, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975