Comparative genomics among Saccharomyces cerevisiae × Saccharomyces kudriavzevii natural hybrid strains isolated from wine and beer reveals different origins
Open Access
- 20 August 2012
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Genomics
- Vol. 13 (1), 407
- https://doi.org/10.1186/1471-2164-13-407
Abstract
Background: Interspecific hybrids between S. cerevisiae × S. kudriavzevii have frequently been detected in wine and beer fermentations. Significant physiological differences among parental and hybrid strains under different stress conditions have been evidenced. In this study, we used comparative genome hybridization analysis to evaluate the genome composition of different S. cerevisiae × S. kudriavzevii natural hybrids isolated from wine and beer fermentations to infer their evolutionary origins and to figure out the potential role of common S. kudriavzevii gene fraction present in these hybrids. Results: Comparative genomic hybridization (CGH) and ploidy analyses carried out in this study confirmed the presence of individual and differential chromosomal composition patterns for most S. cerevisiae × S. kudriavzevii hybrids from beer and wine. All hybrids share a common set of depleted S. cerevisiae genes, which also are depleted or absent in the wine strains studied so far, and the presence a common set of S. kudriavzevii genes, which may be associated with their capability to grow at low temperatures. Finally, a maximum parsimony analysis of chromosomal rearrangement events, occurred in the hybrid genomes, indicated the presence of two main groups of wine hybrids and different divergent lineages of brewing strains. Conclusion: Our data suggest that wine and beer S. cerevisiae × S. kudriavzevii hybrids have been originated by different rare-mating events involving a diploid wine S. cerevisiae and a haploid or diploid European S. kudriavzevii strains. Hybrids maintain several S. kudriavzevii genes involved in cold adaptation as well as those related to S. kudriavzevii mitochondrial functions.This publication has 44 references indexed in Scilit:
- Microbe domestication and the identification of the wild genetic stock of lager-brewing yeastProceedings of the National Academy of Sciences of the United States of America, 2011
- Aneuploidy confers quantitative proteome changes and phenotypic variation in budding yeastNature, 2010
- Remarkably ancient balanced polymorphisms in a multi-locus gene networkNature, 2010
- Chimeric Genomes of Natural Hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzeviiApplied and Environmental Microbiology, 2009
- Genome Sequence of the Lager Brewing Yeast, an Interspecies HybridDNA Research, 2009
- Fragile Genomic Sites Are Associated with Origins of ReplicationGenome Biology and Evolution, 2009
- Comparative genomics of wild type yeast strains unveils important genome diversityBMC Genomics, 2008
- Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianusGenome Research, 2008
- Molecular Characterization of New Natural Hybrids of Saccharomyces cerevisiae and S . kudriavzevii in BrewingApplied and Environmental Microbiology, 2008
- Natural Populations of Saccharomyces kudriavzevii in Portugal Are Associated with Oak Bark and Are Sympatric with S. cerevisiae and S. paradoxusApplied and Environmental Microbiology, 2008