Reconstruction of the genome origins and evolution of the hybrid lager yeast Saccharomyces pastorianus
- 11 September 2008
- journal article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 18 (10), 1610-1623
- https://doi.org/10.1101/gr.076075.108
Abstract
Inter-specific hybridization leading to abrupt speciation is a well-known, common mechanism in angiosperm evolution; only recently, however, have similar hybridization and speciation mechanisms been documented to occur frequently among the closely related group of sensu stricto Saccharomyces yeasts. The economically important lager beer yeast Saccharomyces pastorianus is such a hybrid, formed by the union of Saccharomyces cerevisiae and Saccharomyces bayanus-related yeasts; efforts to understand its complex genome, searching for both biological and brewing-related insights, have been underway since its hybrid nature was first discovered. It had been generally thought that a single hybridization event resulted in a unique S. pastorianus species, but it has been recently postulated that there have been two or more hybridization events. Here, we show that there may have been two independent origins of S. pastorianus strains, and that each independent group—defined by characteristic genome rearrangements, copy number variations, ploidy differences, and DNA sequence polymorphisms—is correlated with specific breweries and/or geographic locations. Finally, by reconstructing common ancestral genomes via array-CGH data analysis and by comparing representative DNA sequences of the S. pastorianus strains with those of many different S. cerevisiae isolates, we have determined that the most likely S. cerevisiae ancestral parent for each of the independent S. pastorianus groups was an ale yeast, with different, but closely related ale strains contributing to each group’s parentage.Keywords
This publication has 48 references indexed in Scilit:
- Molecular Characterization of New Natural Hybrids of Saccharomyces cerevisiae and S . kudriavzevii in BrewingApplied and Environmental Microbiology, 2008
- A burst of protein sequence evolution and a prolonged period of asymmetric evolution follow gene duplication in yeastGenome Research, 2007
- Expanded protein information at SGD: new pages and proteome browserNucleic Acids Research, 2007
- The Stanford Microarray Database: implementation of new analysis tools and open source release of softwareNucleic Acids Research, 2006
- NCBI GEO standards and services for microarray dataNature Biotechnology, 2006
- Pure and Mixed Genetic Lines of Saccharomyces bayanus and Saccharomyces pastorianus and Their Contribution to the Lager Brewing Strain GenomeApplied and Environmental Microbiology, 2006
- MUSCLE: multiple sequence alignment with high accuracy and high throughputNucleic Acids Research, 2004
- Sequencing and comparison of yeast species to identify genes and regulatory elementsNature, 2003
- Life with 6000 GenesScience, 1996
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990