Human placental lipid induces melanogenesis through p38 MAPK in B16F10 mouse melanoma
- 9 March 2005
- journal article
- Published by Wiley in Pigment Cell Research
- Vol. 18 (2), 113-121
- https://doi.org/10.1111/j.1600-0749.2005.00219.x
Abstract
Melanogenesis is one of the characteristic functional activities of melanocyte/melanoma and is regulated via mitogen-activated protein kinase (MAPK) and Akt/protein kinase B (PKB) pathways. Placental total lipid fraction (PTLF), prepared from a hydroalcoholic extract of fresh term human placenta contains sphingolipids and was recently shown to stimulate melanogenesis via up-regulation of the key enzyme tyrosinase in B16F10 mouse melanoma cells. How such lipids mediate their effects on pigmentation and tyrosinase expression is a particularly important aspect of melanogenesis. To study the signaling that leads to tyrosinase expression, we have investigated the roles of the MAPK and Akt/PKB pathways in B16F10 melanoma cells in melanogenesis in response to PTLF. Treatment of cells with PTLF led to the time dependent phosphorylation of p38 MAPK. SB203580, a p38 MAPK inhibitor, completely blocked the PTLF-induced melanogenesis by inhibiting promoter activity and subsequent expression of tyrosinase. Phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002 a blocker of the Akt signaling pathway, or an inhibitor of MEK (MAPK/ERK Kinase), PD98059 when included along with PTLF was found to potentiate PTLF-induced phosphorylation of p38 MAPK together with tyrosinase expression and melanogenesis. The results suggest that the activation of p38 MAPK plays a crucial role in PTLF-induced B16F10 melanogenesis by up-regulating tyrosinase expression.Keywords
This publication has 42 references indexed in Scilit:
- Lounging in a lysosome: the intracellular lifestyle of Coxiella burnetiiCellular Microbiology, 2007
- De Novo Ceramide Synthesis Participates in the Ultraviolet B Irradiation-Induced Apoptosis in Undifferentiated Cultured Human KeratinocytesJournal of Investigative Dermatology, 2003
- Microphthalmia Transcription Factor Is a Target of the p38 MAPK Pathway in Response to Receptor Activator of NF-κB Ligand SignalingJournal of Biological Chemistry, 2002
- The involvement of p38 mitogen‐activated protein kinase in the α‐melanocyte stimulating hormone (α‐MSH)‐induced melanogenic and anti‐proliferative effects in B16 murine melanoma cellsFEBS Letters, 2000
- The Regulation and Activities of the Multifunctional Serine/Threonine Kinase Akt/PKBExperimental Cell Research, 1999
- Regulatory Effects of Heat on Normal Human Melanocyte Growth and Melanogenesis: Comparative Study with UVBJournal of Investigative Dermatology, 1998
- Inhibition of the Phosphatidylinositol 3-Kinase/p70S6-Kinase Pathway Induces B16 Melanoma Cell DifferentiationJournal of Biological Chemistry, 1996
- DNA damage and melanogenesisNature, 1994
- Ultraviolet radiation directly induces pigment production by cultured human melanocytesJournal of Cellular Physiology, 1987
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970