Physical manipulation of calcium oscillations facilitates osteodifferentiation of human mesenchymal stem cells
- 30 January 2007
- journal article
- research article
- Published by Wiley in The FASEB Journal
- Vol. 21 (7), 1472-1480
- https://doi.org/10.1096/fj.06-7153com
Abstract
The role of cytosolic calcium oscillation has long been recognized in the regulation of cellular and molecular interactions. Information embedded in calcium oscillation can provide molecular cues for cell behavior such as cell differentiation. Although calcium dynamics are versatile and likely to depend on the cell type, the calcium dynamics in human mesenchymal stem cells (hMSCs) and its role in differentiation are yet to be fully elucidated. In the present study we characterized the calcium oscillation profiles in hMSCs before and after subjecting the cells to the osteoinductive factors. Our findings indicate that the calcium spikes decreased rapidly with osteodifferentiation to a level observed in terminally differentiated human osteoblasts. In addition, the calcium oscillations appear to serve as a bidirectional signal during hMSC differentiation. While an altered calcium oscillation pattern may be an indicator for hMSC differentiation, it is also likely to be involved in directing hMSC differentiation. Treatment of hMSCs with a noninvasive electrical stimulation, for example, not only altered the calcium oscillations but also facilitated osteodifferentiation. Regulation of calcium oscillation by external physical stimulation could amplify hMSC differentiation into a tissue-specific lineage and may offer an alternate biotechnology to harness the unique properties of stem cells.Keywords
Funding Information
- National Institutes of Health (GM060741, EB006067)
- Office of Naval Research (N00014-06-1-0100)
This publication has 36 references indexed in Scilit:
- Physiologic electrical stimulation provokes intracellular calcium increase mediated by phospholipase C activation in human osteoblastsThe FASEB Journal, 2004
- Human Fibroblast Migration in Three-Dimensional Collagen Gel in Response to Noninvasive Electrical Stimulus. II. Identification of Electrocoupling Molecular MechanismsTissue Engineering, 2004
- Human Fibroblast Migration in Three-Dimensional Collagen Gel in Response to Noninvasive Electrical Stimulus. I. Characterization of Induced Three-Dimensional Cell MovementTissue Engineering, 2004
- Functional expression of Ca2+ signaling pathways in mouse embryonic stem cellsCell Calcium, 2004
- Mesenchymal stem cells: clinical applications and biological characterizationThe International Journal of Biochemistry & Cell Biology, 2004
- A review of electrocoupling mechanisms mediating facilitated wound healingIEEE Transactions on Plasma Science, 2002
- Calcium Oscillation Linked to Pacemaking of Interstitial Cells of CajalOnline Journal of Public Health Informatics, 2002
- Sensing the environment: a historical perspective on integrin signal transductionNature, 2002
- Ca2+ and BMP-6 Signaling Regulate E2F during Epidermal Keratinocyte DifferentiationOnline Journal of Public Health Informatics, 2001
- Acceleration of experimental endochondral ossification by biophysical stimulation of the progenitor cell poolJournal of Orthopaedic Research, 1996