Acceleration of experimental endochondral ossification by biophysical stimulation of the progenitor cell pool
- 1 July 1996
- journal article
- research article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 14 (4), 582-589
- https://doi.org/10.1002/jor.1100140412
Abstract
Endochondral ossification can be modulated by a number of biochemical and biophysical stimuli. This study uses the experimental model of decalcified bone matrix-induced endochondral ossification to examine the effect of one biophysical stimulus, an electromagnetic field, on chondrogenesis, calcification, and osteogenesis. A temporal acceleration and quantitative increase in sulfate incorporation, glycosaminoglycan content, and calcification suggests that the stimulation of endochondral ossification is due to an increase in extracellular matrix synthesis. The locus of that stimulation is identified in the mesenchymal stage of endo-chondral bone development, and stimulation at this stage is essential for accelerated bone formation. The data suggest that enhanced differentiation of mesenchymal stem cells present at this stage is most likely responsible for the increase in extracellular matrix synthesis and bone maturation.Keywords
This publication has 41 references indexed in Scilit:
- Influence of electromagnetic fields on endochondral bone formationJournal of Cellular Biochemistry, 1993
- Mesenchymal stem cellsJournal of Orthopaedic Research, 1991
- Nonthermal 60 Hz sinusoidal magnetic‐field exposure enhances 45Ca2+ uptake in rat thymocytes: dependence on mitogen activationFEBS Letters, 1990
- Non-Invasive Low-Intensity Pulsed Ultrasound Accelerates Bone Healing in the RabbitJournal of Orthopaedic Trauma, 1990
- Current Concepts of Fracture HealingClinical Orthopaedics and Related Research, 1989
- Extremely low frequency pulsed electromagnetic fields increase interleukin‐2 (IL‐2) utilization and IL‐2 receptor expression in mitogen‐stimulated human lymphocytes from old subjectsFEBS Letters, 1989
- Controlled Mechanical Stimulation in the Treatment of Tibial FracturesPublished by Ovid Technologies (Wolters Kluwer Health) ,1989
- The effects of demineralized bone matrix and direct current on an “in vivo” culture of bone marrow cellsJournal of Orthopaedic Research, 1989
- Influence of electromagnetic fields on the efflux of calcium ions from brain tissue in vitro: A three‐model analysis consistent with the frequency response up to 510 HzBioelectromagnetics, 1988
- Fracture healing in the rabbit fibula when subjected to various capacitively coupled electrical fieldsJournal of Orthopaedic Research, 1985