Electrochemical Aptamer-Based Sandwich Assays for the Detection of Explosives
- 21 April 2012
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 84 (10), 4245-4247
- https://doi.org/10.1021/ac300606n
Abstract
Electrochemical impedance spectroscopy (EIS) is used to detect 2,4,6-trinitrotoluene (TNT) in a novel sandwiched structure which relies on the specific interactions between (i) primary amine with TNT and (ii) TNT and anti-TNT aptamer. With pure targets, the assay has a sensitivity of 10–14 M, a dynamic range of 10–14–10–3 M, and employs a small sample volume (25 μL). The method’s sensitivity is comparable to state of the art optical methods with the added advantages of electrochemical detection, which can be easily miniaturized and implemented into a hand-held device.Keywords
This publication has 15 references indexed in Scilit:
- Supersensitive Detection of Explosives by Silicon Nanowire ArraysAngewandte Chemie-International Edition, 2010
- Detection of Molecular Interactions with Modified Ferrocene Self-Assembled MonolayersThe Journal of Physical Chemistry B, 2010
- High Specificity, Electrochemical Sandwich Assays Based on Single Aptamer Sequences and Suitable for the Direct Detection of Small-Molecule Targets in Blood and Other Complex MatricesJournal of the American Chemical Society, 2009
- Biosensor-based on-site explosives detection using aptamers as recognition elementsAnalytical and Bioanalytical Chemistry, 2008
- Selective detection of gas-phase TNT by integrated optical waveguide spectrometry using molecularly imprinted sol–gel sensing filmsAnalytica Chimica Acta, 2007
- Sensors—An effective approach for the detection of explosivesJournal of Hazardous Materials, 2007
- On-line electrochemical monitoring of (TNT) 2,4,6-trinitrotoluene in natural watersAnalytica Chimica Acta, 2003
- Single-Channel Microchip for Fast Screening and Detailed Identification of Nitroaromatic Explosives or Organophosphate Nerve AgentsAnalytical Chemistry, 2002
- Micromachined Electrophoresis Chips with Electrochemical Detectors for Analysis of Explosive Compounds in Soil and GroundwaterEnvironmental Science & Technology, 2000
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990