MC3T3-E1 cell response to microporous tantalum oxide surfaces enriched with Ca, P and Mg
- 1 May 2021
- journal article
- research article
- Published by Elsevier BV in Materials Science and Engineering: C
- Vol. 124, 112008
- https://doi.org/10.1016/j.msec.2021.112008
Abstract
The formation of a porous oxide surface doped with osteoconductive elements, Ca, P and Mg, to enhance osseointegration, was achieved through micro arc oxidation. Micro arc oxidation parameters, such as electrolyte composition, concentration and applied voltage, were studied to understand their effect on the morphology and chemical composition of the samples surface.& nbsp; Considering the optimum atomic concentration reported in literature for each osteoconductive element, microporous Ta anodic oxide samples treated with calcium acetate (CaA) and 13-glycerophosphate (13-GP) revealed that an increase of 13-GP molarity in the electrolyte boosts Ca incorporation, as well as, increasing the porosity. In adding magnesium acetate (MgA) to the electrolyte, when composed by CaA + 13-GP, both addition and variation of MgA did not affect the surface morphology along the samples, being incorporated into the oxide layer for 0.1 M.& nbsp; Finally, in vitro tests were carried out to study the biocompatibility of Ta, to verify the cytotoxicity of the samples and their behavior towards cells, by performing adhesion and differentiation tests with the MC3T3-E1 cell line. Cytotoxicity tests revealed that the samples were non-toxic. Despite none of the samples having been raised up through cell adhesion tests, cell differentiation revealed promising results for the Ta-CaP.Keywords
Funding Information
- Agência Nacional de Inovação SA
- EU Framework Programme for Research and Innovation Marie Sklodowska-Curie Actions
- Fundação para a Ciência e a Tecnologia
This publication has 50 references indexed in Scilit:
- The impact of fabrication conditions on the quality of Au nanoparticle arrays on dimpled Ta templatesNanotechnology, 2012
- Amorphous Calcium Orthophosphates: Nature, Chemistry and Biomedical ApplicationsInternational Journal of Materials and Chemistry, 2012
- Synthesis and properties of hydroxyapatite-containing porous titania coating on ultrafine-grained titanium by micro-arc oxidationActa Biomaterialia, 2010
- Bone sialoprotein plays a functional role in bone formation and osteoclastogenesisThe Journal of Experimental Medicine, 2008
- Titanium oxide films produced on commercially pure titanium by anodic oxidation with different voltagesMaterials Characterization, 2006
- Porous Structure Characterization in Titanium Coating for Surgical ImplantsMaterials Research, 2002
- Thin tantalum and tantalum oxide films grown by pulsed laser depositionApplied Surface Science, 2000
- XPS study of some calcium compoundsJournal of the American Academy of Dermatology, 1995
- The Human Bone Sialoprotein Gene (IBSP): Genomic Localization and CharacterizationGenomics, 1993
- Double anodization experiments in tantalumElectrochimica Acta, 1985