Capsid-Dependent and -Independent Postentry Restriction of Primate Lentivirus Tropism in Rodent Cells
Open Access
- 15 January 2004
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (2), 1006-1011
- https://doi.org/10.1128/jvi.78.2.1006-1011.2004
Abstract
Retrovirus tropism can be restricted by cellular factors such as Fv1, Ref1, and Lv1 that inhibit infection by targeting the incoming viral capsid. Here, we show that rodent cells exhibit differential sensitivity to infection by vesicular stomatitis virus G-pseudotyped lentiviruses and that differences between human immunodeficiency virus type 1 and simian immunodeficiency virus (SIVmac) infectivity are sometimes, but not always, governed by determinants in capsid-p2. In at least one case, resistance to SIVmac infection could be eliminated by saturation of target cells with noninfectious SIVmac particles. However, cross-saturation experiments and analysis of Fv1 -null cells engineered to express natural or artificial Fv1 proteins revealed that lentivirus restriction in mouse cells is independent of Fv1. Overall, these findings indicate that novel restriction factors in rodents can modulate sensitivity to specific primate lentiviruses.Keywords
This publication has 31 references indexed in Scilit:
- Human and Simian Immunodeficiency Virus Capsid Proteins Are Major Viral Determinants of Early, Postentry Replication Blocks in Simian CellsJournal of Virology, 2003
- Abrogation of Ref1 Retrovirus Restriction in Human CellsJournal of Virology, 2002
- Susceptibility of Rat-Derived Cells to Replication by Human Immunodeficiency Virus Type 1Journal of Virology, 2001
- Mouse-Human Heterokaryons Support Efficient Human Immunodeficiency Virus Type 1 AssemblyJournal of Virology, 2001
- Use of a Transient Assay for Studying the Genetic Determinants of Fv1 RestrictionJournal of Virology, 2000
- Positional cloning of the mouse retrovirus restriction gene FvlNature, 1996
- Early replication block of human immunodeficiency virus type 1 in monkey cellsJournal of General Virology, 1995
- Loss of Fv-1 restriction in Balb/3T3 cells following infection with a single N tropic murine leukemia virus particleCell, 1977
- A MAJOR GENETIC LOCUS AFFECTING RESISTANCE TO INFECTION WITH MURINE LEUKEMIA VIRUSESThe Journal of Experimental Medicine, 1971
- Susceptibility to Two Strains of Friend Leukemia Virus in MiceScience, 1967