Sub-second isoelectric focusing in free flow using a microfluidic device
- 7 October 2003
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Lab on a Chip
- Vol. 3 (4), 224-227
- https://doi.org/10.1039/b308476k
Abstract
Using a microfabricated chip with a bed volume of 0.2 µL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.Keywords
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