Effect of Rubus idaeus Extracts in Murine Chondrocytes and Explants
Open Access
- 9 February 2021
- journal article
- research article
- Published by MDPI AG in Biomolecules
- Vol. 11 (2), 245
- https://doi.org/10.3390/biom11020245
Abstract
Osteoarthritis is characterized by cartilage loss resulting from the activation of chondrocytes associated with a synovial inflammation. Activated chondrocytes promote an increased secretion of matrix proteases and proinflammatory cytokines leading to cartilage breakdown. Since natural products possess anti-inflammatory properties, we investigated the direct effect of Rubus idaeus extracts (RIE) in chondrocyte metabolism and cartilage loss. The effect of RIE in chondrocyte metabolism was analyzed in murine primary chondrocytes and cartilage explants. We also assessed the contribution of RIE in an inflammation environment by culturing mice primary chondrocytes with the supernatant of Raw 264.7 macrophage-like cells primed with RIE. In primary chondrocytes, RIE diminished chondrocyte hypertrophy (Col10), while increasing the expression of catabolic genes (Mmp-3, Mmp-13) and reducing anabolic genes (Col2a1, Acan). In cartilage explants, Rubus idaeus prevented the loss of proteoglycan (14.84 ± 3.07% loss of proteoglycans with IL1 alone vs. 3.03 ± 1.86% with IL1 and 100 µg/mL of RIE), as well as the NITEGE neoepitope expression. RIE alone reduced the expression of Il1 and Il6 in macrophages, without changes in Tnf and Cox2 expression. The secretome of macrophages pre-treated with RIE and transferred to chondrocytes decreases the gene and protein expression of Mmp-3 and Cox2. In conclusion, these data suggest that RIE may protect from chondrocyte catabolism and cartilage loss in inflammatory conditions. Further evaluations are need before considering RIE as a candidate for the treatment for osteoarthritis.Keywords
This publication has 26 references indexed in Scilit:
- Loss of sclerostin promotes osteoarthritis in mice via β-catenin-dependent and -independent Wnt pathwaysArthritis Research & Therapy, 2015
- Decreased histone deacetylase 4 is associated with human osteoarthritis cartilage degeneration by releasing histone deacetylase 4 inhibition of runt-related transcription factor-2 and increasing osteoarthritis-related genes: a novel mechanism of human osteoarthritis cartilage degenerationArthritis Research & Therapy, 2014
- Dkk-1-Mediated Inhibition of Wnt Signaling in Bone Ameliorates Osteoarthritis in MiceArthritis & Rheumatology, 2014
- Human osteoarthritic synovium impacts chondrogenic differentiation of mesenchymal stem cells via macrophage polarisation stateOsteoarthritis and Cartilage, 2014
- Rubus idaeus L. reverses epithelial-to-mesenchymal transition and suppresses cell invasion and protease activities by targeting ERK1/2 and FAK pathways in human lung cancer cellsFood and Chemical Toxicology, 2013
- Kaempferol inhibits IL-1β-induced proliferation of rheumatoid arthritis synovial fibroblasts and the production of COX-2, PGE2 and MMPsInternational Journal of Molecular Medicine, 2013
- Biologic agents in osteoarthritis: hopes and disappointmentsNature Reviews Rheumatology, 2013
- Synovial inflammation, immune cells and their cytokines in osteoarthritis: a reviewOsteoarthritis and Cartilage, 2012
- Anti-inflammatory Effects of Polyphenolic-Enriched Red Raspberry Extract in an Antigen-Induced Arthritis Rat ModelJournal of Agricultural and Food Chemistry, 2011
- Crosstalk between cartilage and bone: When bone cytokines matterCytokine & Growth Factor Reviews, 2011