Comparison of manual and automated nucleic acid extraction from whole‐blood samples
- 1 January 2007
- journal article
- research article
- Published by Wiley in Journal of Clinical Laboratory Analysis
- Vol. 21 (4), 244-248
- https://doi.org/10.1002/jcla.20174
Abstract
Nucleic acid extraction and purification from whole blood is a routine application in many laboratories. Automation of this procedure promises standardized sample treatment, a low error rate, and avoidance of contamination. The performance of the BioRobot M48 (Qiagen) and the manual QIAmp® DNA Blood Mini Kit (Qiagen) was compared for the extraction of DNA from whole blood. The concentration and purity of the extracted DNAs were determined by spectrophotometry. Analytical sensitivity was assessed by common PCR and genotyping techniques. The quantity and quality of the generated DNAs were slightly higher using the manual extraction method. The results of downstream applications were comparable to each other. Amplification of high‐molecular‐weight PCR fragments, genotyping by restriction digest, and pyrosequencing were successful for all samples. No cross‐contamination could be detected. While automated DNA extraction requires significantly less hands‐on time, it is slightly more expensive than the manual extraction method. J. Clin. Lab. Anal. 21:244–248, 2007.Keywords
This publication has 10 references indexed in Scilit:
- Optimization and validation of a fully automated silica-coated magnetic beads purification technology in forensicsForensic Science International, 2005
- A Simple Automated Instrument for DNA Extraction in Forensic CaseworkJournal of Forensic Sciences, 2005
- Successful amplification of extremely GC-rich promoter regions using a novel ???slowdown PCR??? techniquePharmacogenetics, 2003
- An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinaseInternational journal of legal medicine, 2003
- Comparison of Automated and Manual Nucleic Acid Extraction Methods for Detection of Enterovirus RNAJournal of Clinical Microbiology, 2003
- Prediction of successful weight reduction under sibutramine therapy through genotyping of the G-protein ??3 subunit gene (GNB3) C825T polymorphismPharmacogenetics, 2003
- Rapid Detection of the CYP2D6*3, CYP2D6*4, and CYP2D6*6 Alleles by Tetra-Primer PCR and of the CYP2D6*5 Allele by Multiplex Long PCRClinical Chemistry, 2000
- Association of a human G-protein β3 subunit variant with hypertensionNature Genetics, 1998
- Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid PurityBioTechniques, 1997