Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria×ananassa)
Open Access
- 1 May 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 60 (7), 2093-2106
- https://doi.org/10.1093/jxb/erp085
Abstract
The gene encoding stilbene synthase is frequently used to modify plant secondary metabolism with the aim of producing the self-defence phytoalexin resveratrol. In this study, strawberry (Fragaria×ananassa) was transformed with the NS-Vitis3 gene encoding stilbene synthase from frost grape (Vitis riparia) under the control of the cauliflower mosaic virus 35S and the floral filament-specific fil1 promoters. Changes in leaf metabolites were investigated with UPLC-qTOF-MS (ultra performance liquid chromatography-quadrupole time of flight mass spectrometry) profiling, and increased accumulation of cinnamate, coumarate, and ferulate derivatives concomitantly with a decrease in the levels of flavonols was observed, while the anticipated resveratrol or its derivatives were not detected. The changed metabolite profile suggested that chalcone synthase was down-regulated by the genetic modification; this was verified by decreased chalcone synthase transcript levels. Changes in the levels of phenolic compounds led to increased susceptibility of the transgenic strawberry to grey mould fungus.Keywords
This publication has 64 references indexed in Scilit:
- Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and StructureTHE PLANT CELL ONLINE, 2007
- A comprehensive and engaging overview of the type III family of polyketide synthasesCurrent Opinion in Chemical Biology, 2007
- RNA Interference Silencing of Chalcone Synthase, the First Step in the Flavonoid Biosynthesis Pathway, Leads to Parthenocarpic Tomato FruitsPlant Physiology, 2007
- Nuclear Magnetic Resonance Spectroscopy-Based Metabolite Profiling of Transgenic Tomato Fruit Engineered to Accumulate Spermidine and Spermine Reveals Enhanced Anabolic and Nitrogen-Carbon InteractionsPlant Physiology, 2006
- High Content of Resveratrol in Lettuce Transformed with a Stilbene Synthase Gene ofParthenocissus henryanaJournal of Agricultural and Food Chemistry, 2006
- Piceid (Resveratrol Glucoside) Synthesis in Stilbene Synthase Transgenic Apple FruitJournal of Agricultural and Food Chemistry, 2006
- Expression profiling reveals off-target gene regulation by RNAiNature Biotechnology, 2003
- Nontargeted Metabolome Analysis by Use of Fourier Transform Ion Cyclotron Mass SpectrometryOMICS: A Journal of Integrative Biology, 2002
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- Metabolic Profiling Allows Comprehensive Phenotyping of Genetically or Environmentally Modified Plant SystemsPlant Cell, 2001