High Content of Resveratrol in Lettuce Transformed with a Stilbene Synthase Gene ofParthenocissus henryana

Abstract

Resveratrol (trans-3,5,4‘-trihydroxystilbene) is a plant phytoalexin which has positive effects on human health. Stilbene synthase (STS) is a key enzyme involved in resveratrol biosynthesis. To construct a vector for STS expression in lettuce plant, a cDNA-encoding STS of Parthenocissus henryana was fused to the Cauliflower mosaic virus (CaMV) 35S promoter, and the bar gene was used as a selective marker gene. To increase the expression of STS, the expression cassette was flanked by MARs. In transgenic lettuce plants, an additional compound was identified as resveratrol by HPLC and ESI-MS. Quantitative analysis showed that the average content of resveratrol reached 56.40 ± 5.52μg/g leaf fresh weight, which was comparable to the amount in grape skin. Anticancer assay in HeLa cells revealed that apoptosis was induced by 200 μM of resveratrol extracted from transgenic lettuce. Keywords: Lettuce; resveratrol; stilbene synthase (STS); MARs; Parthenocissus henryana