Insulin‐specific T cells are a predominant component of islet infiltrates in pre‐diabetic NOD mice
- 1 August 1994
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 24 (8), 1853-1857
- https://doi.org/10.1002/eji.1830240820
Abstract
Numerous investigations have demonstrated that T cells are involved in destruction of β cells in the NOD mouse, a widely studied model of type I diabetes. In this report we describe a series of islet-specific T cell lines established from islet-infiltrating lymphocytes obtained from individual pre-diabetic NOD mice as well as a large panel of clones derived from these lines. Proliferation assays indicated that these nominally islet-specific lines responded vigorously to porcine insulin. Furthermore, of 40 islet-specific clones derived from lines established from 12-week-old mice, 22 (55%) responded to insulin. A similar analysis of islet-specific clones established from 7-week-old mice indicated that 2 of 14 (14%) were insulin specific. These findings demonstrate that insulin-specific T cells can comprise a major portion of the spontaneously arising T cell response to islets in NOD mice.Keywords
This publication has 24 references indexed in Scilit:
- Oral tolerance to myelin basic protein and natural recovery from experimental autoimmune encephalomyelitis are associated with downregulation of inflammatory cytokines and differential upregulation of transforming growth factor beta, interleukin 4, and prostaglandin E expression in the brain.The Journal of Experimental Medicine, 1992
- Studies on autoimmunity for T-cell-mediated beta-cell destruction. Distinct difference in beta-cell destruction between CD4+ and CD8+ T-cell clones derived from lymphocytes infiltrating the islets of NOD miceDiabetes, 1992
- T cell receptor V gene usage of islet beta cell-reactive T cells is not restricted in non-obese diabetic mice.The Journal of Experimental Medicine, 1991
- Acceleration of Diabetes in Young Nod Mice with a CD4 + Islet-specific T Cell CloneScience, 1990
- An explanation for the protective effect of the MHC class II I–E molecule in murine diabetesNature, 1989
- Type I diabetes in NOD mice is not associated with insulin-specific, autoreactive T cellsJournal of Autoimmunity, 1989
- Syngeneic transfer of autoimmune diabetes from diabetic NOD mice to healthy neonates. Requirement for both L3T4+ and Lyt-2+ T cells.The Journal of Experimental Medicine, 1987
- Preventive effect of monoclonal anti-L3T4 antibody on development of diabetes in NOD miceDiabetes, 1987
- Characterization of the two nonallelic genes encoding mouse preproinsulinJournal of Molecular Evolution, 1986
- The structure and evolution of the two nonallelic rat preproinsulin genesCell, 1979