Regulation of Interleukin 8 Production and Gene Expression in Human Adipose Tissue in Vitro1
Open Access
- 1 March 2001
- journal article
- other
- Published by The Endocrine Society in Journal of Clinical Endocrinology & Metabolism
- Vol. 86 (3), 1267-1273
- https://doi.org/10.1210/jcem.86.3.7264
Abstract
A variety of cytokines and other compounds are produced in the human adipose tissue and may have autocrine functions in the adipose tissue as well as be involved in the complications seen in association with obesity. Because it recently has been reported that interleukin 8 (IL-8), through its effects on the macrophage and endothelial cell, may be involved in the pathogenesis of atherosclerosis, we found it of interest to investigate whether IL-8 is produced in human adipose tissue in vitro. Human sc adipose tissue was investigated both in incubations with whole adipose tissue fragments as well as with isolated mature adipocytes. In adipose tissue fragments, IL-1β (3 nm) and tumor necrosis factor α (0.6 nm) were able to stimulate IL-8 production by 12-fold and 5-fold, respectively (P < 0.001), when incubated for 48 h. Incubations with isolated adipocytes were performed up to 6 h, and IL-1β and tumor necrosis factor α significantly increased IL-8 production by 50–60% (P < 0.05). Dexamethasone (50 nm) decreased IL-8 production from adipose tissue fragments by 57% (P < 0.01) and from adipocytes by 37% (P < 0.05). IL-8 messenger RNA expression in adipocytes incubated with IL-1β was increased already after 2 h (P < 0.05). Thus, the effect of proinflammatory cytokines and dexamethasone on IL-8 production in adipose tissue seems to be mediated at the transcriptional level. In conclusion, it is demonstrated for the first time that IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications.Keywords
This publication has 25 references indexed in Scilit:
- Subcutaneous adipose tissue expression of tumour necrosis factor‐α is not associated with whole body insulin resistance in obese nondiabetic or in type‐2 diabetic subjectsEuropean Journal of Clinical Investigation, 2000
- Adipose tissue as an endocrine and paracrine organInternational Journal of Obesity, 1998
- Obesity and diabetes in TNF-alpha receptor- deficient mice.JCI Insight, 1998
- Elevation of Plasma Cytokines in Disorders of Excessive Daytime Sleepiness: Role of Sleep Disturbance and ObesityJournal of Clinical Endocrinology & Metabolism, 1997
- Macrophages Isolated From Human Atherosclerotic Plaques Produce IL-8, and Oxysterols May Have a Regulatory Function for IL-8 ProductionArteriosclerosis, Thrombosis, and Vascular Biology, 1997
- Effects of an Engineered Human Anti–TNF-α Antibody (CDP571) on Insulin Sensitivity and Glycemic Control in Patients With NIDDMDiabetes, 1996
- Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance.JCI Insight, 1995
- Interleukin-6 stimulates hepatic triglyceride secretion in rats.Endocrinology, 1995
- Adipose Expression of Tumor Necrosis Factor-α: Direct Role in Obesity-Linked Insulin ResistanceScience, 1993
- Release and effects of prostaglandins in adipose tissueProstaglandins, Leukotrienes & Essential Fatty Acids, 1992