Macrophages Isolated From Human Atherosclerotic Plaques Produce IL-8, and Oxysterols May Have a Regulatory Function for IL-8 Production
- 1 February 1997
- journal article
- research article
- Published by Ovid Technologies (Wolters Kluwer Health) in Arteriosclerosis, Thrombosis, and Vascular Biology
- Vol. 17 (2), 317-323
- https://doi.org/10.1161/01.atv.17.2.317
Abstract
Oxysterols are biologically active molecules generated during oxidation of LDL. Several of these oxysterols were found in macrophage-derived foam cells from human atherosclerotic tissue (eg, 7-hydroxycholesterol, 7-ketocholesterol, 5-epoxycholesterol, and 25-hydroxycholesterol). A specific stimulation of interleukin-8 (IL-8) production by oxidized LDL (oxLDL) has been shown by other investigators. In foam cells from human atherosclerotic tissue, we found high levels of IL-8 (183.1 pg/10 6 cells) compared with monocytes (23.2 pg/10 6 cells) or monocyte-derived macrophages in culture (1.5 pg/10 6 cells). When monocytes and monocyte-derived macrophages, in vitro, were exposed to a series of different oxysterols, we found that all oxysterols tested had a tendency to stimulate IL-8 production but that 25-hydroxycholesterol was the most potent one. This stimulation of IL-8 production was time and dose dependent and could be blocked by cycloheximide. These results indicate that oxysterols in oxLDL may have a regulatory effect on IL-8 production. IL-8, a potent chemoattractant, may play a role in the recruitment of T lymphocytes and smooth muscle cells into the subendothelial space and may contribute to the formation of atherosclerotic lesions.Keywords
This publication has 29 references indexed in Scilit:
- Oxysterols present in atherosclerotic tissue decrease the expression of lipoprotein lipase messenger RNA in human monocyte-derived macrophages.JCI Insight, 1996
- Lipids and oxidised lipids in human atherosclerotic lesions at different stages of developmentBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1995
- The pathogenesis of atherosclerosis: a perspective for the 1990sNature, 1993
- Pathogenesis of Atherosclerotic LesionsCardiology in Review, 1993
- Isolation of cell populations from arterial tissue, using monoclonal antibodies and magnetic microspheresAtherosclerosis, 1991
- Properties of the Novel Proinflammatory Supergene "Intercrine" Cytokine FamilyAnnual Review of Immunology, 1991
- Regulation of the mevalonate pathwayNature, 1990
- Aortic intimal lipid content and serum lipoproteins in patients undergoing coronary by-pass surgery as related to clinical prognosisAtherosclerosis, 1988
- 25-Hydroxycholesterol-induced elevations in45Ca uptake: Permeability changes in P815 cellsJournal of Cellular Physiology, 1985
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976