A cryogenic direct-plotting system for fabrication of 3D collagen scaffolds for tissue engineering
- 12 October 2009
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of Materials Chemistry
- Vol. 19 (46), 8817-8823
- https://doi.org/10.1039/b914187a
Abstract
The goal of tissue engineering is to repair or regenerate damaged tissue using a combination of cellular biology and materials engineering techniques. One of the challenging problems in tissue engineering is the development of a reproducible three-dimensional (3D) scaffold to support cell migration and infiltration. Although natural polymers, such as dissolved collagen or alginate, are considered ideal for this purpose, their hydrophilic properties have hindered the fabrication of designed 3D scaffold structures. To overcome this problem, we developed a novel system for the cryogenic plotting of 3D scaffolds. Using this technique, we created various 3D collagen scaffolds with designed pore structures that exhibited desired properties. The diameter of the individual collagen strands, which varied from 250 µm to 500 µm, was reproducibly dependent on processing parameters, and the final collagen scaffold showed little shrinkage (less than 12%) relative to the initial design. To evaluate the fabricated scaffold, we adapted the scaffold to regenerate skin tissue. Immunohistochemical analysis demonstrated that co-cultured keratinocytes and fibroblasts completely migrated throughout the 3D collagen scaffold and keratinocytes were well differentiated on the surface of scaffold like a human skin.Keywords
This publication has 39 references indexed in Scilit:
- Erratum to: SnapShot: Polymer Scaffolds for Tissue Engineering [Biomaterials 30/4 (2009) 701–702]Biomaterials, 2009
- Physical approaches to biomaterial designNature Materials, 2009
- Novel 3D collagen scaffolds fabricated by indirect printing technique for tissue engineeringJournal of Biomedical Materials Research Part B: Applied Biomaterials, 2007
- Functionalization of poly(L‐lactide) nanofibrous scaffolds with bioactive collagen moleculesJournal of Biomedical Materials Research Part A, 2007
- Porosity of 3D biomaterial scaffolds and osteogenesisBiomaterials, 2005
- Porous scaffold design for tissue engineeringNature Materials, 2005
- Fibroblast reaction to island topography: changes in cytoskeleton and morphology with timeBiomaterials, 2003
- Biodegradable Polymer Scaffolds with Well-Defined Interconnected Spherical Pore NetworkTissue Engineering, 2001
- Tissue EngineeringScience, 1993
- Haemopoietic long‐term bone marrow cultures from adult mice show osteogenic capacity in vitro on 3–dimensional collagen spongesCell Proliferation, 1992