Recombinant Mycobacterium tuberculosis protein associated with mammalian cell entry
Open Access
- 1 April 2001
- journal article
- research article
- Published by Hindawi Limited in Cellular Microbiology
- Vol. 3 (4), 247-254
- https://doi.org/10.1046/j.1462-5822.2001.00110.x
Abstract
The ability to gain entry and resist the antimicrobial intracellular environment of mammalian cells is an essential virulence property of Mycobacterium tuberculosis. A purified recombinant protein expressed by a 1362 bp locus (mce1) in the M. tuberculosis genome promoted uptake into HeLa cells of polystyrene latex microspheres coated with the protein. N-terminus deletion constructs of Mce1 identified a domain located between amino acid positions 106 and 163 that was needed for this cell uptake activity. Mce1 contained hydrophobic stretches at the N-terminus predictive of a signal sequence, and colloidal gold immunoelectron microscopy indicated that the corresponding native protein is expressed on the surface of the M. tuberculosis organism. The complete M. tuberculosis genome sequence revealed that it contained four homologues of mce (mce1, mce2, mce3, mce4) and that they were all located within operons composed of genes arranged similarly at different locations in the chromosome. Recombinant Mce2, which had the highest level of identity (67%) to Mce1, was unable to promote the association of microspheres with HeLa cells. Although the exact function of Mce1 is still unknown, it appears to serve as an effector molecule expressed on the surface of M. tuberculosis that is capable of eliciting plasma membrane perturbations in non-phagocytic mammalian cells.Keywords
This publication has 12 references indexed in Scilit:
- Analysis of the proteome of Mycobacterium tuberculosis in silicoTubercle and Lung Disease, 1999
- Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequenceNature, 1998
- Bacterial entry into epithelial cells: the paradigm of ShigellaTrends in Microbiology, 1996
- The Yersinia YpkA Ser/Thr kinase is translocated and subsequently targeted to the inner surface of the HeLa cell plasma membraneMolecular Microbiology, 1996
- Molecular genetic bases of Salmonella entry into host cellsMolecular Microbiology, 1996
- Cell‐surface‐bound Yersinia translocate the protein tyrosine phosphatase YopH by a polarized mechanism into the target cellMolecular Microbiology, 1995
- Cloning of an M. tuberculosis DNA fragment associated with entry and survival inside cellsScience, 1993
- Signal transduction in the mammalian cell during bacterial attachment and entryCell, 1993
- A COMPARISON OF THE GROWTH OF SELECTED MYCOBACTERIA IN HELA, MONKEY KIDNEY, AND HUMAN AMNION CELLS IN TISSUE CULTUREThe Journal of Experimental Medicine, 1958
- GROWTH CHARACTERISTICS OF TUBERCLE BACILLI AND CERTAIN OTHER MYCOBACTERIA IN HELA CELLSThe Journal of Experimental Medicine, 1957