Enhanced Expression ofdltABCDIs Associated with the Development of Daptomycin Nonsusceptibility in a Clinical Endocarditis Isolate ofStaphylococcus aureus
Open Access
- 15 December 2009
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 200 (12), 1916-1920
- https://doi.org/10.1086/648473
Abstract
Using isogenic clinical bloodstream Staphylococcus aureus strains from a patient with relapsing endocarditis, we investigated the transcriptional profiles of the mprF and dlt genes in the context of cell-surface charge and daptomycin nonsusceptibility. As in prior studies, a point mutation within mprF was observed in the daptomycin-nonsusceptible strain. However, neither the transcriptional profile of mprF nor the membrane phospholipid analyses were compatible with the anticipated mprF gain-in-function phenotype. In contrast, we demonstrated enhanced dlt expression coincident with increased positive surface charge and reduced daptomycin bindingKeywords
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