A LysR-Type Regulator, CidR, Is Required for Induction of the Staphylococcus aureus cidABC Operon
- 1 September 2005
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 187 (17), 5893-5900
- https://doi.org/10.1128/jb.187.17.5893-5900.2005
Abstract
The Staphylococcus aureus cidABC and lrgAB operons have been shown to regulate murein hydrolase activity and affect antibiotic tolerance. The cid operon enhances murein hydrolase activity and antibiotic sensitivity, whereas the lrg operon inhibits these processes. Based on these findings and the structural similarities of the cidA and lrgA gene products to the bacteriophage holin family of proteins, we have proposed that the cid and lrg operons encode holin- and antiholin-like proteins, respectively, that function to control the murein hydrolase activity produced by the bacteria. Analysis of cid operon transcription revealed the presence of two transcripts, one spanning all three cid genes and whose expression is induced by growth in the presence of acetic acid and the other spanning cidB and cidC only that is produced in a sigma B-dependent manner. The cidABC operon lies immediately downstream from the cidR gene, encoding a potential LysR-type transcriptional regulator. In this study, we demonstrate that cidR is involved in the regulation of cidABC expression. Northern blot analyses revealed that the cidR gene product positively regulates cidABC expression by increasing transcription in the presence of acetic acid produced as a result of the metabolism of glucose. As expected for an operon that encodes a positive effector of murein hydrolase activity, the upregulation of cidABC expression resulted in increased murein hydrolase activity produced by these cells. Furthermore, it was demonstrated that antibiotic tolerance and stationary-phase survival of S. aureus are affected by the cidR gene. Taken together, these results demonstrate that the cidR gene product functions as a transcriptional activator of cidABC transcription in response to acetic acid accumulation in the growth medium.Keywords
This publication has 26 references indexed in Scilit:
- The Staphylococcus aureus cidC gene encodes a pyruvate oxidase that affects acetate metabolism and cell death in stationary phaseMolecular Microbiology, 2005
- Are the molecular strategies that control apoptosis conserved in bacteria?Trends in Microbiology, 2003
- The Staphylococcus aureus cidAB Operon: Evaluation of Its Role in Regulation of Murein Hydrolase Activity and Penicillin ToleranceJournal of Bacteriology, 2003
- Analysis of Catabolite Control Protein A-Dependent Repression in Staphylococcus xylosus by a Genomic Reporter Gene SystemJournal of Bacteriology, 2001
- Functional Dissection of the LysR-type CysB Transcriptional RegulatorPublished by Elsevier BV ,2001
- MOLECULAR BIOLOGY OF THE LysR FAMILY OF TRANSCRIPTIONAL REGULATORSAnnual Review of Microbiology, 1993
- Improved method for electroporation of Staphylococcus aureusFEMS Microbiology Letters, 1992
- High efficiency transformation of Escherichia coli with plasmidsGene, 1990
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- Multiple Antibiotic Resistance in a Bacterium with Suppressed Autolytic SystemNature, 1970